The Knockout Mouse Project

By C. Austin, J. Battey, A. Bradley, Maja Bucan, M. Capecchi, F. S. Collins, W. Dove, G. Duyk, S. Dymecki, Janan T. Eppig and 31 more. Nature Genetics, 2004

618 citations recorded by the source corpus.

Mouse knockout technology provides a powerful means of elucidating gene function in vivo, and a publicly available genome-wide collection of mouse knockouts would be significantly enabling for biomedical discovery. To date, published knockouts exist for only about 10% of mouse genes. Furthermore, many of these are limited in utility because they have not been made or phenotyped in standardized ways, and many are not freely available to researchers. It is time to harness new technologies and efficiencies of production to mount a high-throughput international effort to produce and phenotype knockouts for all mouse genes, and place these resources into the public domain.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/c4aaaeed311d92b1fb0183a8a90f8f913f52cb2b

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In Genetics

1. A. Holmes
2. A. Hopkins
3. Shizuo Akira
4. J. Cryan

In Molecular Biology

1. A. Holmes
2. M. Hayden
3. S. Ghosh
4. Shizuo Akira
5. Cynthia L. Smith
6. K. Matsumoto
7. A. Hopkins
8. Janan T. Eppig

Citations in the corpus

• The Mammalian Phenotype Ontology: enabling robust annotation and comparative analysis (2009, background): “…Mouse Knockout Consortium projects, US Knockout Mouse Project (KOMP), North American Conditional Mouse Mutagenesis (NORCOMM), European Conditional Mouse Mutagenesis (EUCOMM), and Texas A&M Institute for Genomic Medicine (TIGM), 5–8 much of which will only be available in electronic form.”
• The Mammalian Phenotype Ontology as a tool for annotating, analyzing and comparing phenotypic information (2004, background): “Current international efforts to 'make a mutation' for every gene through mutagenesis [1] and genetic engineering [2,3] make it imperative for phenotype data to be represented in standard descriptive formats to enable computational analysis and comparison.”
• TAK1, but not TAB1 or TAB2, plays an essential role in multiple signaling pathways in vivo. (2005, methodology): “To explore the biological function of TAK1 in vivo, we generated TAK1-deficient (Tak1) mice by microinjecting gene trap-mutated Tak1 embryonic stem cells (ES) into blastocysts of C57BL/6 mice (Fig. 1A; Stryke et al. 2003; Austin et al. 2004; Skarnes et al. 2004).”
• Emerging functional and mechanistic paradigms of mammalian long non-coding RNAs (2012, background): “It is also critical to point out that mouse knockouts of many protein-coding genes are also viable and display no obvious phenotypes (32).”
• Model organisms: The ascent of mouse: advances in modelling human depression and anxiety (2005, positive): “For example, methods originally developed in model organisms such as Caenorhabditis elegans or Drosophila melanogaster that allow for the generation of random mutations, such as gene trapping and chemical mutagenesis, are being applied to the mouse to study behaviou”
• Network pharmacology: the next paradigm in drug discovery. (2008, background): “in the mouse genome and profile each knockout across a battery of phenotypic assays has revealed that as few as 10% of knockouts demonstrate phenotypes that may be of value for drug target validatio”