Degradation and Mineralization of High-Molecular-Weight Polycyclic Aromatic Hydrocarbons by Defined Fungal-Bacterial Cocultures
By S. Boonchan, M. Britz, G. Stanley. Applied and Environmental Microbiology, 2000
680 citations recorded by the source corpus.
ABSTRACT This study investigated the biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons (PAHs) in liquid media and soil by bacteria (Stenotrophomonas maltophilia VUN 10,010 and bacterial consortium VUN 10,009) and a fungus (Penicillium janthinellum VUO 10,201) that were isolated from separate creosote- and manufactured-gas plant-contaminated soils. The bacteria could use pyrene as their sole carbon and energy source in a basal salts medium (BSM) and mineralized significant amounts of benzo[a]pyrene cometabolically when pyrene was also present in BSM. P. janthinellum VUO 10,201 could not utilize any high-molecular-weight PAH as sole carbon and energy source but could partially degrade these if cultured in a nutrient broth. Although small amounts of chrysene, benz[a]anthracene, benzo[a]pyrene, and dibenz[a,h]anthracene were degraded by axenic cultures of these isolates in BSM containing a single PAH, such conditions did not support significant microbial growth or PAH mineralization. However, significant degradation of, and microbial growth on, pyrene, chrysene, benz[a]anthracene, benzo[a]pyrene, and dibenz[a,h]anthracene, each as a single PAH in BSM, occurred when P. janthinellum VUO 10,201 and either bacterial consortium VUN 10,009 or S. maltophilia VUN 10,010 were combined in the one culture, i.e., fungal-bacterial cocultures: 25% of the benzo[a]pyrene was mineralized to CO2 by these cocultures over 49 days, accompanied by transient accumulation and disappearance of intermediates detected by high-pressure liquid chromatography. Inoculation of fungal-bacterial cocultures into PAH-contaminated soil resulted in significantly improved degradation of high-molecular-weight PAHs, benzo[a]pyrene mineralization (53% of added [14C]benzo[a]pyrene was recovered as14CO2 in 100 days), and reduction in the mutagenicity of organic soil extracts, compared with the indigenous microbes and soil amended with only axenic inocula.
Subfields: Health, Toxicology and Mutagenesis, Pollution
View Paper: https://www.semanticscholar.org/paper/c3beebfbc3d500935b14df551f756c24ae885c7e
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Citations in the corpus
- Isolation and Selection of a Highly Tolerant Microbial Consortium with Potential for PAH Biodegradation from Heavy Crude Oil-Contaminated Soils (2014, neutral): “…Pseudomonas sp. (Avramova et al. 2008), Enterobacter sp. (Bautista et al. 2009), Stenothrophomonas sp. (Bautista et al. 2009), S. maltophilia (Boonchan et al. 2000), Bacillus sp. (Wong et al. 2004), B. cereus (Kazunga and Aitken 2000), Streptomyces sp. (Chaudhary et al. 2011), C. glutamicum…”
- Current State of Knowledge in Microbial Degradation of Polycyclic Aromatic Hydrocarbons (PAHs): A Review (2016, background): “It has been seen that, inoculation of fungal–bacterial co-cultures into the soils contaminated with PAHs, can significantly enhance degradation of HMW PAHs, like chrysene, benzo-[a]anthracene and dibenzo[a,h]anthracene (Boonchan et al., 2000).”
- Bacterial-Fungal Interactions: Hyphens between Agricultural, Clinical, Environmental, and Food Microbiologists (2011, background): “(49) compared the degradations of pyrene, benzo[a]pyrene, and dibenz[a,h]anthracene in monocultures of Penicillium janthinellum and Stenotrophomonas maltophilia and in cocultures of the two.”
- Microbial biodegradation of polyaromatic hydrocarbons. (2008, background): “Inoculation of fungal–bacterial cocultures into PAHcontaminated soil resulted in significantly improved degradation of some high-MW PAHs, such as chrysene, benzo[a]anthracene and dibenzo[a,h]anthracene (Boonchan et al., 2000).”
- Biodegradation ability and dioxgenase genes of PAH-degrading Sphingomonas and Mycobacterium strains isolated from mangrove sediments. (2010, background): “Co-metabolism was an important degradation process in situation where the larger and often more recalcitrant HMW PAHs are present in mixtures with the more readily degraded and smaller LMW PAHs (Bouchez et al., 1995; Boonchan et al., 2000; Hwang and Cutright, 2002; Pizzul et al., 2006).”
- Isolation and characterization of engine oil degrading indigenous microrganisms in Kwazulu-Natal, South Africa (2007, background): “Used motor oil contains more metals and heavy polycyclic aromatic hydrocarbons (PAHs) that would contribute to chronic hazards including mutagenicity and carcinogenicity (Keith and Telliard, 1979; Hagwell et al., 1992; Boonchan et al., 2000).”
- Degradation of polyaromatic hydrocarbons by Burkholderia cepacia 2A-12 (2003, background): “Some laboratory-scale tests to demonstrate the potential for bioaugmentation in PHA-contaminated soils have been conducted ( Boonchan et al. 2000; Canet et al. 2001).”
- Recent Advances in Petroleum Microbiology (2003, neutral): “In this case, a consortium of the four strains did not enhance phenanthrene mineralization, as has been observed in other studies with defined bacterial and bacterial-fungal consortia (61, 67, 101, 616).”