Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture
By Anca M. Pașca, S. Sloan, L. Clarke, Yuan Tian, Christopher D. Makinson, Nina Huber, C. Kim, Jin-Young Park, N. O’Rourke, K. Nguyen and 5 more. Nature Methods, 2015
1,453 citations recorded by the source corpus.
The human cerebral cortex develops through an elaborate succession of cellular events that, when disrupted, can lead to neuropsychiatric disease. The ability to reprogram somatic cells into pluripotent cells that can be differentiated in vitro provides a unique opportunity to study normal and abnormal corticogenesis. Here, we present a simple and reproducible 3D culture approach for generating a laminated cerebral cortex–like structure, named human cortical spheroids (hCSs), from pluripotent stem cells. hCSs contain neurons from both deep and superficial cortical layers and map transcriptionally to in vivo fetal development. These neurons are electrophysiologically mature, display spontaneous activity, are surrounded by nonreactive astrocytes and form functional synapses. Experiments in acute hCS slices demonstrate that cortical neurons participate in network activity and produce complex synaptic events. These 3D cultures should allow a detailed interrogation of human cortical development, function and disease, and may prove a versatile platform for generating other neuronal and glial subtypes in vitro.
Subfields: Gender Studies, Language and Linguistics
View Paper: https://www.semanticscholar.org/paper/c3105332bd70bd90a3136215a00f117e583273eb
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Language and Linguistics
Citations in the corpus
- Complex oscillatory waves emerging from cortical organoids model early human brain network development (2019, background): “Organoids generated from induced pluripotent stem cells (iPSCs) have emerged as a scaled-down and three-dimensional model of the human brain, mimicking various developmental features at the cellular and molecular levels (Camp et al., 2015; Cederquist et al., 2019; Giandomenico et al., 2019; Lancaste”
- Midbrain-like organoids from human pluripotent stem cells contain functional dopaminergic and neuromelanin producing neurons (2016, background): “(Figure 4A) (Paşca et al., 2015; Yuan et al., 2015).”
- A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity. (2019, background): “Recently, 3D organoid culture systems have been developed that capture the phenotypic and molecular heterogeneity found in various organs (Clevers, 2016), including cerebral organoids (Kadoshima et al., 2013; Lancaster et al., 2013; Paşca et al., 2015; Qian et al., 2016).”
- Brain Region-specific Organoids using Mini-bioreactors for Modeling ZIKV Exposure (2016, positive): “Several pioneering studies showed that cerebral organoid systems offer improved growth conditions for 3D tissue, leading to a more representative model of the developing human brain (Danjo et al., 2011; Kadoshima et al., 2013; Lancaster et al., 2013; Mariani et al., 2015; Pasca et al., 2015).”
- Brain organoids: advances, applications and challenges (2019, background): “Neuronal function and activity Excitatory and inhibitory neurons in cortical organoids are functional, but unsurprisingly less mature, compared to adult neurons (Birey et al., 2017; Pasca et al., 2015; Qian et al., 2016).”