Complex oscillatory waves emerging from cortical organoids model early human brain network development

By Cleber A. Trujillo, Richard Gao, P. Negraes, Jing Gu, J. Buchanan, Sebastian Preissl, Allen Wang, Wei Wu, G. Haddad, I. Chaim and 6 more. Cell Stem Cell, 2019

769 citations recorded by the source corpus.

SUMMARY Structural and transcriptional changes during early brain maturation follow fixed developmental programs defined by genetics. However, whether this is true for functional network activity remains unknown, primarily due to experimental inaccessibility of the initial stages of the living human brain. Here, we developed human cortical organoids that dynamically change cellular populations during maturation, and exhibited consistent increases in electrical activity over the span of several months. The spontaneous network formation displayed periodic and regular oscillatory events that were dependent on glutamatergic and GABAergic signaling. The oscillatory activity transitioned to more spatiotemporally irregular patterns, while synchronous network events resembled features similar to those observed in preterm human electroencephalography. These results show that the development of structured network activity in a human neocortex model may follow stable genetic programming. Our approach provides opportunities for investigating and manipulating the role of network activity in the developing human cortex.

Subfields: Biomedical Engineering, Cellular and Molecular Neuroscience, Cognitive Neuroscience

View Paper: https://www.semanticscholar.org/paper/5f78f5783ce3284e15d49d581081211d84d59c99

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cognitive Neuroscience

  1. Ángela Hoyo
  2. Josué Rico-Picó
  3. M. R. Rueda
  4. Sebastián Moyano
  5. M. Á. Ballesteros-Duperón
  6. Á. Conejero
  7. M. Rothbart

Citations in the corpus