Can genetic manipulation of plant nitrogen assimilation enzymes result in increased crop yield and greater N-use efficiency? An assessment

By M. Andrews, P. Lea, J. Raven, K. Lindsey. 2004

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Nitrogen Assimilation and its Relevance to Crop Improvement (2010, background): “A number of species of plants have been transformed with genes important in N metabolism, and the effect of these transformed genes has been measured on a range of characters, including NUE (see Andrews et al., 2004; Good et al., 2004; Lea & Azevedo, 2007, for further discussion).”
Do plants need nitrate? The mechanisms by which nitrogen form affects plants (2013, background): “These results have potential implications for agricultural systems as NO3− is the main form of N available to crops in cultivated soil and many crops assimilate substantial NO3− in the shoot (Andrews et al., 2004).”
Nitrogen fixation in legumes and actinorhizal plants in natural ecosystems: values obtained using 15N natural abundance (2011, background): “Where tested, legumes and actinorhizal plants capable of N2 fixation have been found to be able to utilise or have the enzymes required to utilise soil NH4+ and NO3− (e.g. Sutherland et al. 1985; Andrews et al. 1984, 1990, 2004; Vogel and Dawson 1991; Troelstra et al. 1992; Woodall and Forde 1996).”