How pure are your vesicles?

By J. Webber, Aled Clayton. Journal of Extracellular Vesicles, 2013

753 citations recorded by the source corpus.

We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA-assay. Such an approach is simple enough to apply to every vesicle preparation within a given laboratory, assisting researchers as a routine quality control step. Also, the approach may aid in comparing/standardising vesicle purity across diverse studies, and may be of particular importance in evaluating vesicular biomarkers. We herein propose some criteria to aid in the definition of pure vesicles.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/bf75afc95b0ae97047d18d132796ba019b5cdf77

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In Molecular Biology

Citations in the corpus

Focus on Extracellular Vesicles: Introducing the Next Small Big Thing (2016, background): “Finally, specific purification protocols after ultracentrifugation not only affect EV purity and yield, but often lead to protein loss in the preparation [163,164].”
Optimized exosome isolation protocol for cell culture supernatant and human plasma (2015, methodology): “Density gradient purification is a technique repeatedly shown to provide the highest degree of purity (6,12,13).”
Methods for Separation and Characterization of Extracellular Vesicles: Results of a Worldwide Survey Performed by the ISEV Rigor and Standardization Subcommittee (2020, background): “EV separation: Several years after the 2015 survey [8], ultracentrifugation remains the most common EV separation technique, despite potential drawbacks such as aggregation and incomplete separation [15,16].”
The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling (2014, methodology): “2 mm filters to obtain the CCM, and (b) no extra washing step was carried out in regard to recently published observations (11).”
Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey (2016, positive): “EVs isolated by ultracentrifugation are known to suffer from non-vesicular macromolecule contamination (9).”