Methods for Separation and Characterization of Extracellular Vesicles: Results of a Worldwide Survey Performed by the ISEV Rigor and Standardization Subcommittee
By F. Royo, C. Théry, Juan Manuel Falcón-Pérez, Rienk Nieuwland, Kenneth W. Witwer. Cells, 2020
346 citations recorded by the source corpus.
Research on extracellular vesicles (EVs) is growing exponentially due to an increasing appreciation of EVs as disease biomarkers and therapeutics, an expanding number of EV-containing materials under study, and application of new preparation, detection, and cargo analysis methods. Diversity of both sources and methodologies imposes challenges on the comparison of measurement results between studies and laboratories. While reference guidelines and minimal requirements for EV research have achieved the important objective of assembling community consensus, it is also essential to understand which methodologies and quality controls are currently being applied, and how usage trends are evolving. As an initial response to this need, the International Society for Extracellular Vesicles (ISEV) performed a worldwide survey in 2015 on “Techniques used for the isolation and characterization of extracellular vesicles” and published the results from this survey in 2016. In 2019, a new survey was performed to assess the changing state of the field. The questionnaire received more than 600 full or partial responses, and the present manuscript summarizes the results of this second worldwide survey. The results emphasize that separation methods such as ultracentrifugation and density gradients are still the most commonly used methods, the use of size exclusion chromatography has increased, and techniques based on tangential flow and microfluidics are now being used by more than 10% of respondents. The survey also reveals that most EV researchers still do not perform sample quality controls before or after isolation of EVs. Finally, the majority of EV researchers emphasize that separation and characterization of EVs should receive more attention.
Subfields: Cancer Research, Infectious Diseases, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/4e86577f9085f9add2acb903dda639c262f8a9de
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Citations in the corpus
- A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option? (2020, methodology): “A follow-up survey conducted in 2019 from 600 respondents by ISEV found that while dUC and density-gradient ultracentrifugation were still the most commonly used methods, the use of SEC has increased markedly, more than double its percent use from 2015 [94].”