Isolation of the Cdc45/Mcm2–7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase

By S. E. Moyer, P. Lewis, M. Botchan. Proceedings of the National Academy of Sciences of the United States of America, 2006

763 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/8971a441c12c6ac65b99eedf56e14d9438f09fa2

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Citations in the corpus

CDK-dependent complex formation between replication proteins Dpb11, Sld2, Pol (epsilon}, and GINS in budding yeast. (2010, background): “…replication proteins found at the replication forks and forms a complex with Cdc45 and Mcm, called the CMG complex in Drosophila embryo extracts (Moyer et al. 2006), the Unwindsome in Xenopus egg extracts (Pacek et al. 2006), and the replisome progression complex (RPC) in budding yeast (Gambus…”
Efficient Initiation of DNA Replication in Eukaryotes Requires Dpb11/TopBP1-GINS Interaction (2013, background): “GINS is a heterotetrameric complex (Sld5-Psf1-Psf2-Psf3) that is loaded onto DNA in the initiation reaction to assemble the CMG complex (7, 8, 23, 24).”
DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7 (2020, background): “Cdc45 and GINS are direct activators of the Mcm2-7 complex and association of these factors is required to form a pair of Cdc45-Mcm2-7-GINS complexes (CMGs), the active eukaryotic replicative helicase (Ilves et al., 2010; Moyer et al., 2006; Pacek et al., 2006).”
Concerted Loading of Mcm2-7 Double Hexamers Around DNA during DNA Replication Origin Licensing (2009, background): “, 2000) as well as eukaryotic Mcm2–7 complexes (Bochman and Schwacha, 2008; Ishimi, 1997; Moyer et al., 2006) on singlestranded DNA substrates need not reflect their mode of action in vivo: even double-stranded DNA translocases like RuvB can function in standard helicase assays (Tsaneva et al.”
Activation of the MCM2-7 Helicase by Association with Cdc45 and GINS proteins (2010, methodology): “The purified MCM2-7 and CMG showed the expected patterns of subunit bands on standard SDS-PAGE (Figure 1B) (Moyer et al., 2006), and the presence of all subunits was verified by mass spectrometry of both the total trypsin digested samples as well as protein bands cut from the SDS-PAGE gel (Figure…”
DONSON facilitates Cdc45 and GINS chromatin association and is essential for DNA replication initiation (2023, background): “Howe v er, the human and Xenopus TopBP1 homologues are much larger proteins, containing eight BRCT domains (1-8), with an additional N-terminal BRCT domain identified in human TopBP1, named BRCT0 ( 51 ).”