In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses
By Y. Itoh, K. Shinya, Maki Kiso, Tokiko Watanabe, Y. Sakoda, M. Hatta, Y. Muramoto, Daisuke Tamura, Yuko Sakai-Tagawa, Saori Sakabe and 41 more. Nature, 2009
1,104 citations recorded by the source corpus.
Influenza A viruses cause recurrent outbreaks at local or global scale with potentially severe consequences for human health and the global economy. Recently, a new strain of influenza A virus was detected that causes disease in and transmits among humans, probably owing to little or no pre-existing immunity to the new strain. On 11 June 2009 the World Health Organization declared that the infections caused by the new strain had reached pandemic proportion. Characterized as an influenza A virus of the H1N1 subtype, the genomic segments of the new strain were most closely related to swine viruses. Most human infections with swine-origin H1N1 influenza viruses (S-OIVs) seem to be mild; however, a substantial number of hospitalized individuals do not have underlying health issues, attesting to the pathogenic potential of S-OIVs. To achieve a better assessment of the risk posed by the new virus, we characterized one of the first US S-OIV isolates, A/California/04/09 (H1N1; hereafter referred to as CA04), as well as several other S-OIV isolates, in vitro and in vivo. In mice and ferrets, CA04 and other S-OIV isolates tested replicate more efficiently than a currently circulating human H1N1 virus. In addition, CA04 replicates efficiently in non-human primates, causes more severe pathological lesions in the lungs of infected mice, ferrets and non-human primates than a currently circulating human H1N1 virus, and transmits among ferrets. In specific-pathogen-free miniature pigs, CA04 replicates without clinical symptoms. The assessment of human sera from different age groups suggests that infection with human H1N1 viruses antigenically closely related to viruses circulating in 1918 confers neutralizing antibody activity to CA04. Finally, we show that CA04 is sensitive to approved and experimental antiviral drugs, suggesting that these compounds could function as a first line of defence against the recently declared S-OIV pandemic.
Subfields: Agronomy and Crop Science, Epidemiology
View Paper: https://www.semanticscholar.org/paper/bb07a85377b9049a050374c8160f5ba1e39893e6
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Agronomy and Crop Science
In Epidemiology
Citations in the corpus
- Structural basis of pre-existing immunity to the 2009 H1N1 pandemic influenza virus (2010, background): “And unlike seasonal flu that usually affects the elderly most severely, swine flu has been primarily targeting people under the age of 65, implying some age-related pre-existing immunity (2, 3).”
- Influenza A viruses: new research developments (2011, neutral): “The slight differences in receptor-binding specificity of the 2009 pandemic strain might partly explain the increased replication, transmission and pathogenesis that were observed in animal models for this virus compared with seasonal viruse”
- Endothelial cells are central orchestrators of cytokine amplification during influenza virus infection (2011, methodology): “To test this, we infected mice with a virulent isolate of the 2009 H1N1 pandemic influenza virus that was isolated from a hospitalized patient and has never been passaged through mice (A/Wisconsin/WSLH34939/09) (Itoh et al., 2009).”