SUMO-1 Conjugation in Vivo Requires Both a Consensus Modification Motif and Nuclear Targeting*
By Manuel S. Rodríguez, C. Dargemont, Ronald T. Hay. Journal of Biological Chemistry, 2001
776 citations recorded by the source corpus.
SUMO-1 is a small ubiquitin-related modifier that is covalently linked to many cellular protein targets. Proteins modified by SUMO-1 and the SUMO-1-activating and -conjugating enzymes are located predominantly in the nucleus. Here we define a transferable sequence containing the ΨKXE motif, where Ψ represents a large hydrophobic amino acid, that confers the ability to be SUMO-1-modified on proteins to which it is linked. Whereas addition of short sequences from p53 and IκBα, containing the ΨKXE motif, to a carrier protein is sufficient for modification in vitro, modification in vivorequires the additional presence of a nuclear localization signal. Thus, protein substrates must be targeted to the nucleus to undergo SUMO-1 conjugation.
Subfields: Immunology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/bac4848dd40d6646b4d3efbfa0313052c5ab0889
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Citations in the corpus
- The SUMO pathway: emerging mechanisms that shape specificity, conjugation and recognition (2010, background): “Consensus motifs for SUMO conjugation Many SUMO-modified proteins identified contain an acceptor Lys within a ψKX(D/E) consensus motif, where ψ is a large hydrophobic residu”
- RNF4 is a poly-SUMO-specific E3 ubiquitin ligase required for arsenic-induced PML degradation (2008, neutral): “d c RNF4 WT mtSIM1 mtSIM2 mtSIM3 mtSIM4 mtSIM1,2 mtSIM1,2,3 mtSIM1,2,3,4 ∆N(1-20) ∆1(31-65) ∆2(66-98) ∆3(99-118) CS1 CS2 SIMs RING”
- Nuclear and unclear functions of SUMO (2003, background): “In fact, a short peptide that contains the ΨKxE motif and a nuclear localization signal (NLS) suffices to produce a SUMO conjugate in vivo”
- GPS-SUMO: a tool for the prediction of sumoylation sites and SUMO-interaction motifs (2014, background): “Previously, experimental studies suggested that most of sumoylation sites follow a canonical consensus motif of –K–X–E ( , a hydrophobic amino acid, such as A, I, L, M, P, F, V or W; X, any amino acid residue) (12,13).”
- Post-translational modifications regulating the activity and function of the nuclear factor kappa B pathway (2006, background): “Moreover, protein sumoylation frequently requires nuclear localization (Rodriguez et al., 2001).”
- Insights in Post-Translational Modifications: Ubiquitin and SUMO (2022, background): “The consensus motif is preferentially targeted for SUMOylation and it is also present in other potential SUMOylation target proteins [131].”
- SUMO and ubiquitin in the nucleus: different functions, similar mechanisms? (2004, background): “A consensus SUMO acceptor site has been identified consisting of the sequence KXE, where is a large hydrophobic amino acid and K is the site of SUMO conjugation (Rodriguez et al. 2001).”