2b-RAD: a simple and flexible method for genome-wide genotyping

By Shi Wang, E. Meyer, John K. McKay, M. Matz. Nature Methods, 2012

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Stacks: an analysis tool set for population genomics (2013, background): “…reads provided by modern sequencing platforms on speciﬁc, restriction enzyme – anchored positions in the genome (e.g. CRoPS, Van Orsouw et al. 2007; RAD-seq, Baird et al. 2008; Etter et al. 2011b; GBS, Elshire et al. 2011; double-digest RAD-seq, Peterson et al. 2012; and 2bRAD, Wang et al. 2012b).”
Stacks 2: Analytical Methods for Paired-end Sequencing Improve RADseq-based Population Genomics (2019, methodology): “Others were designed to minimize primer‐dimers (3RAD; Graham et al., 2015), or to use type IIb restriction enzymes (2bRAD; Wang, Meyer, McKay, & Matz, 2012), or to use biotinylated adapters to extract restriction site‐associated DNA from other genomic DNA (BestRAD), as well as hybrids of the above…”
Genotyping‐by‐Sequencing for Plant Breeding and Genetics (2012, methodology): “…allows nearly all of the restriction sites to be surveyed, and permits marker intensity adjustment POLAND AND RIFE: GENOTYPING -BY-SEQUENCING 95 (Wang et al., 2012). h e next l avor of sequence-based genotyping was multiplexed shotgun genotyping (MSG), which required only one gel purii cation,…”