Genotyping‐by‐Sequencing for Plant Breeding and Genetics

By Jesse Poland, Trevor W. Rife. 2012

830 citations recorded by the source corpus.

Rapid advances in “next‐generation” DNA sequencing technology have brought the US$1000 human (Homo sapiens) genome within reach while providing the raw sequencing output for researchers to revolutionize the way populations are genotyped. To capitalize on these advancements, genotyping‐by‐sequencing (GBS) has been developed as a rapid and robust approach for reduced‐representation sequencing of multiplexed samples that combines genome‐wide molecular marker discovery and genotyping. The flexibility and low cost of GBS makes this an excellent tool for many applications and research questions in plant genetics and breeding. Here we address some of the new research opportunities that are becoming more feasible with GBS. Furthermore, we highlight areas in which GBS will become more powerful with the continued increase of sequencing output, development of reference genomes, and improvement of bioinformatics. The ultimate goal of plant biology scientists is to connect phenotype to genotype. In plant breeding, the genotype can then be used to predict phenotypes and select improved cultivars. Furthering our understanding of the connection between heritable genetic factors and the resulting phenotypes will enable genomics‐assisted breeding to exist on the scale needed to increase global food supplies in the face of decreasing arable land and climate change.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/de841d761a25a3b2eb49895d60b1dfa11913f263

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. Yong‐Bi Fu
2. Y. El-Kassaby
3. M. Gowda
4. Dipnarayan Saha
5. K. Bansal
6. I. Porth
7. Carolee Horbach
8. Yibo Dong

In Molecular Biology

1. Y. El-Kassaby
2. M. Gowda
3. Yong‐Bi Fu
4. I. Porth
5. Carolee Horbach
6. Yibo Dong
7. G. W. Peterson

Citations in the corpus

• Assessment of the Genetic Diversity in Forest Tree Populations Using Molecular Markers (2014, background): “genomes that has involved the genome-wide discovery of SSRs and SNPs and the detection of common, as well as rare functional variants by next generation sequencing [43–49].”
• Genetic and Genomic Resources of Small Millets (2016, background): “It facilitates at the same time with large number of marker discovery at genome-wide scale and efficient genomic selection and genotyping (Poland and Rife, 2012).”
• DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing (2018, neutral): “studies and breeding of diverse crops [100].”
• Genotyping-By-Sequencing for Plant Genetic Diversity Analysis: A Lab Guide for SNP Genotyping (2014, methodology): “First, it combines the processes of marker discovery and genotyping and provides a rapid, high throughput and cost-effective tool for a genome-wide analysis of genetic diversity [5,10].”