Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip'.
By M. Donnelly, G. Luke, A. Mehrotra, Xuejun Li, Lorraine E. Hughes, D. Gani, M. Ryan. Journal of General Virology, 2001
827 citations recorded by the source corpus.
Subfields: Agronomy and Crop Science, Cardiology and Cardiovascular Medicine, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/b77b16712b3bd61994cfc15b0f21723a660e9a28
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In Molecular Biology
Citations in the corpus
- Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide–based retroviral vector (2004, background): “Through a ribosomal skip mechanism, the 2A peptide impairs normal peptide bond formation between the 2A glycine and the 2B proline without affecting the translation of 2”
- Ribosomal frameshifting and transcriptional slippage: From genetic steganography and cryptography to adventitious use (2016, background): “[One of the names for the phenomenon of nascent peptide mediated release, without a stop codon, of upstream encoded protein and continued translation (without initiation) to specify a downstream encoded protein (479) is StopGo (480).”
- High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice (2011, background): “Recent reports demonstrated, however, that ribosomes skip the synthesis of the glycyl-prolyl peptide bond at the C-terminus of a 2A peptide, leading to the cleavage between a 2A peptide and its immediate downstream peptide [5,6,7].”
- Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “This suggests that 2A-cleavage activity and expression of downstream proteins can be modulated by plant developmental stage as well as by upstream proteins, as shown in an in vitro translation system and an animal system (Donnelly et al., 2001; Felipe et al., 2010).”
- Foot-and-Mouth Disease (2004, background): “There has been a suggestion that this cleavage is not a proteolytic event but rather is a modification of the translational machinery by the 2A peptide which allows the release of P1-2A from the ribosome while permitting the synthesis of the downstream proteins to proceed (139, 140).”