Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5

By Ian T. Paulsen, C. Press, Jacques Ravel, D. Kobayashi, G. Myers, D. Mavrodi, R. Deboy, Rekha Seshadri, Q. Ren, R. Madupu and 19 more. Nature Biotechnology, 2005

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Pseudomonas fluorescens Pf-5 is a plant commensal bacterium that inhabits the rhizosphere and produces secondary metabolites that suppress soilborne plant pathogens. The complete sequence of the 7.1-Mb Pf-5 genome was determined. We analyzed repeat sequences to identify genomic islands that, together with other approaches, suggested P. fluorescens Pf-5's recent lateral acquisitions include six secondary metabolite gene clusters, seven phage regions and a mobile genomic island. We identified various features that contribute to its commensal lifestyle on plants, including broad catabolic and transport capabilities for utilizing plant-derived compounds, the apparent ability to use a diversity of iron siderophores, detoxification systems to protect from oxidative stress, and the lack of a type III secretion system and toxins found in related pathogens. In addition to six known secondary metabolites produced by P. fluorescens Pf-5, three novel secondary metabolite biosynthesis gene clusters were also identified that may contribute to the biocontrol properties of P. fluorescens Pf-5.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/b5d9a257c762b225fa6fb1fc9ad3ee371045eaab

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