Full-length RNA-seq from single cells using Smart-seq2

By S. Picelli, O. Faridani, Åsa K. Björklund, Gösta Winberg, Sven Sagasser, Rickard Sandberg. Nature Protocols, 2014

3,760 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/b4e9d1c8a3ea0f6ef37f8936b61e21c19010962d

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In Molecular Biology

Citations in the corpus

Integrated analysis of multimodal single-cell data (2020, methodology): “Samples were then brought into reverse transcription in an adaptation of SMARTseq2 (Picelli et al., 2014) and SCRB-seq (Soumillon et al.”
Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq (2017, methodology): “Libraries from isolated single cells were generated based on the Smart-seq2 protocol (21) with the following modifications.”
COMPREHENSIVE CLASSIFICATION OF RETINAL BIPOLAR NEURONS BY SINGLE-CELL TRANSCRIPTOMICS (2016, methodology): “We performed Smart-seq2 following the published protocol (Picelli et al., 2014) with minor modifications in the reverse transcription (RT) step (Monika Kowalczyk, in preparation).”
Electrical and synaptic integration of glioma into neural circuits (2019, positive): “Cells were sorted into skirted 96-well plates, each well of which contained 4 microliters of lysis buffer as described in Picelli et al. 38 Whole-transcriptome amplification, library construction, sequencing, and processing 96-well plates of single sorted cells were processed using the smart-seq2…”
Single-cell mRNA quantification and differential analysis with Census (2017, background): “Second, most protocols rely on template-switching reverse transcriptases primed at the poly(A) tail of mRNAs and thus generate full-length cDNAs 9 .”
Cellular and inter-cellular rewiring of the human colon during ulcerative colitis (2019, methodology): “After 3 days, spheroids were collected and subjected to bulk RNA-Seq with the SMART-Seq2 protocol (Picelli et al., 2014).”
Stereotypic Immune System Development in Newborn Children (2018, methodology): “Full-length cDNA synthesis and amplification from the polyA+ extracted RNAwas carried out as in Picelli et al. (2014).”
Droplet barcoding for single cell transcriptomics applied to embryonic stem cells (2015, positive): “(Picelli et al. 2014), but are higher than those reported for CEL-Seq (3.4”