Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry

By Hui Zhang, Xiao-Jun Li, Daniel B. Martin, R. Aebersold. Nature Biotechnology, 2003

1,338 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Quantitative, Multiplexed Assays for Low Abundance Proteins in Plasma by Targeted Mass Spectrometry and Stable Isotope Dilution*S (2007, background): “For example, capture of glycosylated proteins or peptides has been shown to greatly reduce plasma complexity and thereby enable detection of some lower abundance proteins (31–35), although recovery may be an issue as peptides from only 100 plasma proteins were detected in studies of serum (32, 33).”
Selected reaction monitoring for quantitative proteomics: a tutorial (2008, methodology): “To be able to quantify low abundance proteins in plasma, peptides with Nlinked sugars were specifically enriched by solid phase capture ( Zhang et al, 2003; Stahl-Zeng et al, 2007).”
Proteomics by mass spectrometry: approaches, advances, and applications. (2009, background): “N-linked glycosylated peptides are selectively bound to the solid support (121) and are subsequently enzymatically released.”