Overexpression of AtMYB44 Enhances Stomatal Closure to Confer Abiotic Stress Tolerance in Transgenic Arabidopsis1[C][W][OA]

By Choonkyun Jung, J. S. Seo, S. Han, Y. Koo, Chung-Ho Kim, S. Song, B. Nahm, Y. Choi, J. Cheong. Plant Physiology, 2007

673 citations recorded by the source corpus.

AtMYB44 belongs to the R2R3 MYB subgroup 22 transcription factor family in Arabidopsis (Arabidopsis thaliana). Treatment with abscisic acid (ABA) induced AtMYB44 transcript accumulation within 30 min. The gene was also activated under various abiotic stresses, such as dehydration, low temperature, and salinity. In transgenic Arabidopsis carrying an AtMYB44 promoter-driven β-glucuronidase (GUS) construct, strong GUS activity was observed in the vasculature and leaf epidermal guard cells. Transgenic Arabidopsis overexpressing AtMYB44 is more sensitive to ABA and has a more rapid ABA-induced stomatal closure response than wild-type and atmyb44 knockout plants. Transgenic plants exhibited a reduced rate of water loss, as measured by the fresh-weight loss of detached shoots, and remarkably enhanced tolerance to drought and salt stress compared to wild-type plants. Microarray analysis and northern blots revealed that salt-induced activation of the genes that encode a group of serine/threonine protein phosphatases 2C (PP2Cs), such as ABI1, ABI2, AtPP2CA, HAB1, and HAB2, was diminished in transgenic plants overexpressing AtMYB44. By contrast, the atmyb44 knockout mutant line exhibited enhanced salt-induced expression of PP2C-encoding genes and reduced drought/salt stress tolerance compared to wild-type plants. Therefore, enhanced abiotic stress tolerance of transgenic Arabidopsis overexpressing AtMYB44 was conferred by reduced expression of genes encoding PP2Cs, which have been described as negative regulators of ABA signaling.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/b2b0004cc8d2cc2f71ca9bac78e6c3eebff22fed

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

In Plant Science

Citations in the corpus

Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “The sections were laser-microdissected using the Veritas Microdissection System LCC1704 (Molecular Devices) and the dissected content was conﬁrmed.”
Gene Expression Profiling of Plants under Salt Stress (2011, background): “…microarray analysis in Arabidopsis under salt stress, it was determined that in transgenic plants overexpressing AtMYB44, a member of the R2R3MYB subgroup 22 transcription factor family, a group of genes encoding serine/threonine protein phosphatases 2C (PP2C) was downregulated (Jung et al., 2008).”
Role of Plant Transcription Factors in Abiotic Stress Tolerance (2011, background): “AtMYB44 TF confers abiotic stress tolerance through enhancing stomatal closure in an ABA-independent manner (Jung et al., 2008).”
Regulation of Transpiration to Improve Crop Water Use (2009, background): “, 2006b), MYB44 that encodes a R2R3 MYB transcription factor (Jung et al., 2008), SNAC1 (Stress-responsive NAC 1) that encodes a member of the NAC (NAM/ATAF/CUC) transcription factor family (Hu et al.”
A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice (2012, background): “Several studies also revealed that MYB proteins in Arabidopsis (AtMYB44, AtMYB60, and AtMYB61) are involved in regulation of stomatal aperture in response to drought stress (Cominelli et al., 2005; Liang et al., 2005; Jung et al., 2008).”
ABA-mediated transcriptional regulation in response to osmotic stress in plants (2011, background): “…have indicated that MYB44 mRNA accumulation is induced in most tissues by a variety of hormone treatments (ABA, IAA, ET, JA and GA), environmental conditions (drought, high salinity, and low temperature), and pathogen infections (Jung et al. 2007, 2008; Kranz et al. 1998; Yanhui et al. 2006).”
Guard Cell Signal Transduction Network: Advances in Understanding Abscisic Acid, CO2, and Ca2+ Signaling (2010, background): “Moreover, transcripts of MYB44, another guard cell–expressed transcription factor, accumulates in response to abiotic stresses (76).”
Recent Advances in Photosynthesis Under Drought and Salinity (2011, background): “under dehydration, salinity, low temperature and ABA treatment (Jung et al., 2008).”