Root-Specific Expression of OsNAC10 Improves Drought Tolerance and Grain Yield in Rice under Field Drought Conditions1[W][OA]

By J. Jeong, Youn Shic Kim, Kwangryul Baek, Harin Jung, Sun-Hwa Ha, Yang Do Choi, Minkyun Kim, C. Reuzeau, Jukon Kim. Plant Physiology, 2010

789 citations recorded by the source corpus.

Drought poses a serious threat to the sustainability of rice (Oryza sativa) yields in rain-fed agriculture. Here, we report the results of a functional genomics approach that identified a rice NAC (an acronym for NAM [No Apical Meristem], ATAF1-2, and CUC2 [Cup-Shaped Cotyledon]) domain gene, OsNAC10, which improved performance of transgenic rice plants under field drought conditions. Of the 140 OsNAC genes predicted in rice, 18 were identified to be induced by stress conditions. Phylogenic analysis of the 18 OsNAC genes revealed the presence of three subgroups with distinct signature motifs. A group of OsNAC genes were prescreened for enhanced stress tolerance when overexpressed in rice. OsNAC10, one of the effective members selected from prescreening, is expressed predominantly in roots and panicles and induced by drought, high salinity, and abscisic acid. Overexpression of OsNAC10 in rice under the control of the constitutive promoter GOS2 and the root-specific promoter RCc3 increased the plant tolerance to drought, high salinity, and low temperature at the vegetative stage. More importantly, the RCc3:OsNAC10 plants showed significantly enhanced drought tolerance at the reproductive stage, increasing grain yield by 25% to 42% and by 5% to 14% over controls in the field under drought and normal conditions, respectively. Grain yield of GOS2:OsNAC10 plants in the field, in contrast, remained similar to that of controls under both normal and drought conditions. These differences in performance under field drought conditions reflect the differences in expression of OsNAC10-dependent target genes in roots as well as in leaves of the two transgenic plants, as revealed by microarray analyses. Root diameter of the RCc3:OsNAC10 plants was thicker by 1.25-fold than that of the GOS2:OsNAC10 and nontransgenic plants due to the enlarged stele, cortex, and epidermis. Overall, our results demonstrated that root-specific overexpression of OsNAC10 enlarges roots, enhancing drought tolerance of transgenic plants, which increases grain yield significantly under field drought conditions.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/221ec9e3b6e6165fbe767820f1fe2bc9b93daa93

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Citations in the corpus

Plant tolerance to drought and salinity: stress regulating transcription factors and their functional significance in the cellular transcriptional network (2011, background): “OsNAC10-regulated target genes mainly included protein kinases and TFs of AP2, WRKY, LRR, NAC, and Zn-finger types as well as the stress-responsive genes cytochrome P450 and the potassium transporter HAK5 ( Jeong et al. 2010 ).”
Genetic engineering and breeding of drought-resistant crops. (2014, background): “Interestingly, with the same strategy, RCc3::OsNAC9 and RCc3::OsNAC10 transgenic rice also showed significantly enhanced DT at the reproductive stage in field trials (45, 92).”
General mechanisms of drought response and their application in drought resistance improvement in plants (2014, background): “Overexpression of OsNAC10 driven by a root-specific promoter RCc3 remarkably enlarged the root diameter in transgenic rice and consequently resulted in enhanced drought tolerance at the reproductive stage, and improved grain yield in the field under both normal and drought stress conditions [172].”
Transcription Factors and Plants Response to Drought Stress: Current Understanding and Future Directions (2016, positive): “Enhanced abiotic stress tolerance in rice caused by overexpressing SNACs was evident when using a root-speciﬁc promoter RCc3 (Jeong et al., 2010, 2013; Redillas et al., 2012).”
The use of JIP test to evaluate drought-tolerance of transgenic rice overexpressing OsNAC10 (2011, background): “The transgenic plants analyzed here were the T6 generation of plants produced and used previously (Jeong et al. 2010; Jang et al. 1999).”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “Most transgenic rice drought studies have been performed under controll d conditi at e rly growth stages, and only limited numbe of studies tions (Du et al., 2010; Hu et al., 2006; Huang et al., 2007; Islam et al., 2009; Jeong et al., 2010; Oh et al., 2009; Xiao et al., 2009; Xiao et al., 2007).”
Engineering Salinity and Water-Stress Tolerance in Crop Plants (2011, background): “However, only the rootspecific overexpression of OsNAC10 (PRCc3::OsNAC10) significantly enhanced drought tolerance at the reproductive stage, increasing grain yield (25–42%) in the field under drought conditions (Jeong et al., 2010).”
The transcriptional regulatory network in the drought response and its crosstalk in abiotic stress responses including drought, cold, and heat (2014, background): “Recent studies have suggested that the root-specific promoter RCc3 is useful for the overexpression of SNACs such as SNAC1 and OsNAC10 to enhance the abiotic stress tolerance of rice in field conditions (Jeong et al., 2010, 2013; Redillas et al., 2012).”