IDENTIFICATION OF BACTERIA ASSOCIATED WITH DINOFLAGELLATES (DINOPHYCEAE) ALEXANDRIUM SPP. USING TYRAMIDE SIGNAL AMPLIFICATION–FLUORESCENT IN SITU HYBRIDIZATION AND CONFOCAL MICROSCOPY1

By Isabelle C. Biegala, G. Kennaway, E. Alverca, Jean‐François Lennon, Daniel Vaulot, N. Simon. 2002

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Application of fluorescent in situ hybridization coupled with tyramide signal amplification (FISH-TSA) to assess eukaryotic picoplankton composition (2002, methodology): “Yet, for cells with a thicker cell wall such as thecate dinoflagellates, Biegala et al. (2002) had no problem with HRP-labeled probe penetration.”
Interactions between Diatoms and Bacteria (2012, background): “Bacterial attachment to living cells in the marine environment has been reported in a number of cases involving a wide range of phytoplankton (20, 62, 90, 91, 121).”
Algae as an important environment for bacteria – phylogenetic relationships among new bacterial species isolated from algae (2013, background): “Proteobacteria and Bacteroidetes are common and in many cases dominant in aquatic environments (Cottrell & Kirchman 2000a, b; Biegala et al. 2002; Simonato et al. 2010); this is proportionally reflected in the discovery of new bacterial taxa from algal sources (together 77% of the 101 valid species…”