Application of fluorescent in situ hybridization coupled with tyramide signal amplification (FISH-TSA) to assess eukaryotic picoplankton composition

By Fabrice Not, N. Simon, Isabelle C. Biegala, Daniel Vaulot. 2002

125 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology, Oceanography

View Paper: https://www.semanticscholar.org/paper/8356b3f188ff0a4a6ffd02a00c9242af1b05307f

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In Ecology

In Molecular Biology

In Oceanography

Citations in the corpus

Composition and temporal variability of picoeukaryote communities at a coastal site of the English Channel from 18S rDNA sequences (2004, methodology): “The use of quantitative approaches such as fluorescent in situ hybridization (FISH; Not et al. 2002) is required to determine the distribution and seasonal dynamics of each group, and representatives of uncultivated groups, especially heterotrophic ones, must be brought into culture to assess their ”
Analysis of photosynthetic picoeukaryote diversity at open ocean sites in the Arabian Sea using a PCR biased towards marine algal plastids (2006, unknown): “Even so, the presence of 2 prymnesiophyte sequences, DYF45 and HE3, closely related to Chrysochromulina sp. from these sites, is consistent with their presence in coastal waters of northwest France.”
Mapping of picoeucaryotes in marine ecosystems with quantitative PCR of the 18S rRNA gene. (2005, methodology): “With cell-based techniques such as FISH, quantification issues are straightforward since it is easy to determine whether a cell is labeled or not, especially when amplification techniques such as TSA are used [42].”
Development of specific rRNA probes to distinguish between geographic clades of the Alexandrium tamarense species complex (2004, unknown): “Instead, other possibilities to enhance the signal to background ratio are the use of microscopy filter sets that partially cut off the autofluorescence, or the use of the Tyramide Signal Amplification system that increases fluorescence intensity 10–20-fold (Not et al., 2002).”
Protistan assemblages across the Indian Ocean, with a specific emphasis on the picoeukaryotes (2008, methodology): “Samples for epifluorescence microscopy were fixed with ice-cold glutaraldehyde (1% final concentration), stained with 4,6-diamidino-2-phenylindole (DAPI; 5mg mL 1 final concentration) and filtered on 0.6mm pore-size black polycarbonate filters.”