Genome-Wide Mapping of the Cohesin Complex in the Yeast Saccharomyces cerevisiae
By Earl F. Glynn, P. Megee, Hong-Guo Yu, Cathy A. Mistrot, Elcin Unal, D. Koshland, J. DeRisi, J. Gerton. PLoS Biology, 2004
448 citations recorded by the source corpus.
In eukaryotic cells, cohesin holds sister chromatids together until they separate into daughter cells during mitosis. We have used chromatin immunoprecipitation coupled with microarray analysis (ChIP chip) to produce a genome-wide description of cohesin binding to meiotic and mitotic chromosomes of Saccharomyces cerevisiae. A computer program, PeakFinder, enables flexible, automated identification and annotation of cohesin binding peaks in ChIP chip data. Cohesin sites are highly conserved in meiosis and mitosis, suggesting that chromosomes share a common underlying structure during different developmental programs. These sites occur with a semiperiodic spacing of 11 kb that correlates with AT content. The number of sites correlates with chromosome size; however, binding to neighboring sites does not appear to be cooperative. We observed a very strong correlation between cohesin sites and regions between convergent transcription units. The apparent incompatibility between transcription and cohesin binding exists in both meiosis and mitosis. Further experiments reveal that transcript elongation into a cohesin-binding site removes cohesin. A negative correlation between cohesin sites and meiotic recombination sites suggests meiotic exchange is sensitive to the chromosome structure provided by cohesin. The genome-wide view of mitotic and meiotic cohesin binding provides an important framework for the exploration of cohesins and cohesion in other genomes.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/b21808ba0528060dd03298baa45aa16cedca6dec
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Citations in the corpus
- Cohesinopathy mutations disrupt the subnuclear organization of chromatin (2009, background): “Cohesin binds to the GAL2 ORF and promoter when the gene is repressed (Glynn et al., 2004).”
- Cohesins functionally associate with CTCF on mammalian chromosome arms. (2008, unknown): “The yeast data are from Lengronne et al., 2004, a slightly lower figure of 84% was given by Glynn et al., 2004.”
- Cohesin: its roles and mechanisms. (2009, background): “Cohesin is enriched in a ∼40-kbp region around centromeres and at specific sites along chromosome arms that frequently correspond to sites of convergent transcription (30, 39, 92).”