SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction

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Inhibitory circuits of the mammalian main olfactory bulb. (2017, background): “…debate emerges from the distinct glomerular organization of PGCs and GCs. Specifically, the limited overlap of homotypic M/TC lateral dendrites (Ke et al. 2013; Kikuta et al. 2013) strongly suggests that homotypic M/TCs primarily interact with distinct populations of GCs and therefore distinct…”
Clarifying Tissue Clearing (2015, neutral): “Sucrose (Tsai et al., 2009b), fructose (Costantini et al., 2015; Ke et al., 2013), glycerol (Meglinski et al., 2002), 2,20-thiodiethanol (TDE) (Aoyagi et al., 2015; Hou et al., 2015; Staudt et al., 2007), and formamide (Kuwajima et al., 2013) have all been used for this purpose.”
Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis. (2014, positive): “…long sample treatment periods (weeks or months) and tissue swelling, were resolved by the development of another clearing reagent, SeeDB, which renders brain samples transparent within a few days by adjusting RI differences between the sample lipids and the surrounding tissue (Ke et al., 2013).”
iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging. (2014, neutral): “One limitation is that the organic solvent it uses rapidly quenches endogenously fluorescent proteins, necessitating prompt imaging if the purpose is to visualize such signals (Ertürk et al., 2014; Ke et al., 2013).”