A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements.

By A. Yang, Marcos Estecio, Ketan Doshi, Y. Kondo, E. Tajara, Jean-Pierre Issa. Nucleic Acids Research, 2004

1,031 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/ae850f5b1caca43c6eb29b64eb0395d67647cc1d

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Citations in the corpus

• Prenatal tobacco smoke exposure affects global and gene-specific DNA methylation. (2009, methodology): “Methylation analyses of LINE1 and AluYb8 were performed by bisulfite-polymerase chain reaction (PCR) pyrosequencing assay using the HotMaster Mix (Eppendorf, Hamburg, Germany) and the PSQ HS 96 Pyrosequencing System (Biotage AB, Uppsala, Sweden) (13).”
• DZNep Is a Global Histone Methylation Inhibitor that Reactivates Developmental Genes Not Silenced by DNA Methylation (2009, methodology): “Quantitation of DNA Methylation by Pyrosequencing Pyrosequencing analysis was done on bisulfite-converted DNA, prepared as described previously (18, 19).”
• Changes in DNA methylation patterns in subjects exposed to low-dose benzene. (2007, methodology): “*DNA methylation of LINE-1 and AluI repeated elements was measured as a surrogate for global DNA methylation (10).”
• Results of a randomized study of 3 schedules of low-dose decitabine in higher-risk myelodysplastic syndrome and chronic myelomonocytic leukemia. (2007, neutral): “(%) 20 mg/m2 intravenously for 5 d 64 (67) 20 mg/m2 subcutaneously for 5 d 14 (15) 10 mg/m2 intravenously for 10 d 17 (18)”
• DNA hypomethylation and human diseases. (2007, methodology): “Because of the high copy number of repeat sequences they offer potentially a much higher technical sensitivity for the detection of aberrantly methylated sequences using PCR-based assays such as those described above [23,202].”
• DNA methylation analysis by pyrosequencing (2007, background): “or global methylation changes by either analyzing the methylation degree in repetitive element”
• Aging and Environmental Exposures Alter Tissue-Specific DNA Methylation Dependent upon CpG Island Context (2009, methodology): “To test the hypothesis that there are associations of age and exposure with methylation, we constructed measures of partial methylation sets, in analogy with global DNA methylation, which is measured on repeats [57].”
• Repurposing the CRISPR-Cas9 system for targeted DNA methylation (2016, methodology): “The LINE– 1 methylation is an established method for assessing global genome methylation (44), although newer and more accurate methods for this purpose, such as reduced representation bisulfite sequencing (RRBS) or methylation arrays, would be more appropriate to rule out any unspecific activity.”
• Analysis of repetitive element DNA methylation by MethyLight (2005, methodology): “COBRA and pyrosequencing (18) platforms showed that Alu or LINE-1 methylation provides a marker for 5-Aza-CdRmediated demethylation in human cancer cell lines.”
• Rapid DNA methylation changes after exposure to traffic particles. (2009, methodology): “4 million Alu repetitive elements, which are heavily methylated (1).”