Oxford Nanopore R10.4 long-read sequencing enables the generation of near-finished bacterial genomes from pure cultures and metagenomes without short-read or reference polishing

By M. Sereika, Rasmus Hansen Kirkegaard, Søren Michael Karst, T. Michaelsen, E. A. Sørensen, R. Wollenberg, Mads Albertsen. Nature Methods, 2022

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Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include short-read polishing to correct insertions and deletions derived from homopolymer regions. Here, we show that Oxford Nanopore R10.4 can be used to generate near-finished microbial genomes from isolates or metagenomes without short-read or reference polishing. This study demonstrates the feasibility of generating near-finished microbial genomes using only Oxford Nanopore R10.4 data from pure cultures or metagenomes.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/ac9375a88ffbe4269cae596ba99cc64057557018