Nutrient limitation as a strategy for increasing starch accumulation in microalgae

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Development of an effective acidogenically digested swine manure-based algal system for improved wastewater treatment and biofuel and feed production (2013, neutral): “Algae growth and their cellular biochemical composition are able to be affected by environmental and culture conditions, such as irradiance, temperature, salinity, and nutrient balance [6,7].”
Effect of light intensity and nitrogen starvation on CO2 fixation and lipid/carbohydrate production of an indigenous microalga Scenedesmus obliquus CNW-N. (2012, background): “…this is not always achievable, because high lipid/carbohydrate contents usually occur under environmental stress (typically nutrient deficiency), which is often associated with relatively low biomass productivity, and thus low overall lipid/carbohydrate productivity (Dragone et al., 2011).”
Morphology, composition, production, processing and applications of Chlorella vulgaris: A review (2014, methodology): “Starch quantification is much better using the enzymatic method compared to the acidic method [118,119].”