From Three-Dimensional Cell Culture to Organs-on-Chips

By D. Huh, G. Hamilton, Donald E. Ingber. Trends in Cell Biology, 2011

1,684 citations recorded by the source corpus.

Three-dimensional (3D) cell culture models have recently garnered great attention because they often promote levels of cell differentiation and tissue organization not possible in conventional two-dimensional (2D) culture systems. Here, we review new advances in 3D culture that leverage microfabrication technologies from the microchip industry and microfluidics approaches to create cell culture microenvironments that both support tissue differentiation and recapitulate the tissue-tissue interfaces, spatiotemporal chemical gradients, and mechanical microenvironments of living organs. These ‘organs-on-chips’ permit study of human physiology in an organ-specific context, enable development of novel in vitro disease models, and could potentially serve as replacements for animals used in drug development and toxin testing.

Subfields: Biomedical Engineering

View Paper: https://www.semanticscholar.org/paper/abbef5515bc6747c7303393a15525273aea34ff7

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In Biomedical Engineering

Citations in the corpus

Three-dimensional bioprinting of thick vascularized tissues (2016, background): “T ability to manufacture human tissues that replicate the essential spatial (1), mechanochemical (2, 3), and temporal aspects of biological tissues (4) would enable myriad applications, including 3D cell culture (5), drug screening (6, 7), disease modeling (8), and tissue repair and regeneration (9,”
Modeling Physiological Events in 2D vs. 3D Cell Culture. (2017, background): “Many of these devices remain 2D in nature, but recently some microfluidic devices have evolved into more 3D-like cultures to mimic organ functions (8, 57).”
Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors (2014, background): “As a result, 2D-cultured cells may not behave as they would in the body because this model does not adequately mimic the in vivo microenvironment.(21) Technologies, such as nano-patterning, which mimics the topographical features of the ECM, have been investigated to improve cellular function and be”
Consensus guidelines for the use and interpretation of angiogenesis assays (2018, background): “Microfabricated devices can provide cultured cells with a microenvironment similar to that in vivo, including the correct ECM (ECM) composition, chemical species, associations with other cells, and mechanical signals to mimic tissue- and organ-level function [204].”
In silico methods and tools for drug discovery (2021, background): “Recent advancements in in vitro models, such as organ-on-chip technology, have accelerated ADME-Tox assessments [4].”
Biochemical analysis on microfluidic chips (2016, background): “3D cell culture on microfluidic chips makes it possible to retain native tissue-specific functions of cells and recapitulate tissue-tissue interfaces, spatiotemporal chemical gradients [16,17].”