Plant DNA flow cytometry and estimation of nuclear genome size.

By J. Doležel, J. Bartoš. Annals of Botany, 2005

1,020 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/ab32f9c562c3431aef25d44bfd15db72a18243f5

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. Johann Greilhuber
2. J. Doležel
3. Ilia J. Leitch
4. T. Ryan Gregory
5. J. van Staden
6. J. Loureiro
7. Michael D. Bennett
8. Todd P. Michael

In Molecular Biology

1. Ilia J. Leitch
2. Johann Greilhuber
3. J. van Staden
4. Todd P. Michael
5. R. Kerstetter
6. Wenqin Wang
7. M. Bairu
8. J. Loureiro

In Plant Science

1. Ilia J. Leitch
2. Johann Greilhuber
3. J. Doležel
4. J. van Staden
5. Michael D. Bennett
6. T. Ryan Gregory
7. Todd P. Michael
8. R. Kerstetter

Citations in the corpus

• Evolution of Genome Size in Duckweeds (Lemnaceae) (2011, methodology): “To extensively estimate the DNA content and to provide a basic reference for duckweed genome sequence research, the nuclear DNA content for 115 different accessions of 23 duckweed species was measured by flow cytometry (FCM) stained with propidium iodide as DNA stain.”
• Plectranthus: A plant for the future? (2011, methodology): “Successful tetraploid treatment was investigated using flow cytometry to measure relative nuclear DNA content (Doležel and Bartoš, 2005).”
• Eukaryotic genome size databases (2006, background): “Recent reviews covering potential problems in genome size estimation include those by Greilhuber (15) for Feulgen densitometry and Dolezel and Bartos (16) for flow cytometry.”
• Genome size, base composition and karyotype of Jatropha curcas L., an important biofuel plant (2008, methodology): “Distributions of fluorescence with high resolution and low CVs were also found by Doležel and Bartoš [31] and Loureiro et al.”
• Somaclonal variation in plants: causes and detection methods (2011, methodology): “Another problem is the absence of a set of internationally agreed DNA reference standards causing limitations in the use of flow cytometry (Doležel and Bartoš 2005).”
• Genetic relations among basil taxa (Ocimum L.) based on molecular markers, nuclear DNA content, and chromosome number (2009, background): “Taxonomy, population biology, and ecology require the analysis of large populations of plants, for which flow cytometry is ideally suited (Doležel and Bartoš 2005).”
• Two new nuclear isolation buffers for plant DNA flow cytometry: a test with 37 species. (2007, methodology): “In some buffers, chelating agents (e.g. EDTA, sodium citrate) are added to bind divalent cations, which serve as cofactors of DNases; inorganic salts (e.g. KCl, NaCl) are used to achieve proper ionic strength (Doležel and Bartoš, 2005).”
• A chromosome-level genome assembly for the Pacific oyster Crassostrea gigas (2020, positive): “To this end, the average genome size was estimated for the 135 sequenced female using the k-mer method [36] and flow cytometry [37].”