Two new nuclear isolation buffers for plant DNA flow cytometry: a test with 37 species.

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The largest eukaryotic genome of them all (2010, methodology): “japonica and the calibration standard were co-chopped in 2 mL of the ‘General purpose isolation buffer’ (Loureiro et al., 2007) with the addition of 3% PVP-40 following the one-step procedure described by Doležel, Greilhuber & Suda (2007).”