A Central Regulatory System Largely Controls Transcriptional Activation and Repression Responses to Phosphate Starvation in Arabidopsis
By Regla Bustos, Gabriel Castrillo, F. Linhares, María Isabel Puga, V. Rubio, Julián Pérez-Pérez, R. Solano, A. Leyva, J. Paz-Ares. PLoS Genetics, 2010
687 citations recorded by the source corpus.
Plants respond to different stresses by inducing or repressing transcription of partially overlapping sets of genes. In Arabidopsis, the PHR1 transcription factor (TF) has an important role in the control of phosphate (Pi) starvation stress responses. Using transcriptomic analysis of Pi starvation in phr1, and phr1 phr1-like (phl1) mutants and in wild type plants, we show that PHR1 in conjunction with PHL1 controls most transcriptional activation and repression responses to phosphate starvation, regardless of the Pi starvation specificity of these responses. Induced genes are enriched in PHR1 binding sequences (P1BS) in their promoters, whereas repressed genes do not show such enrichment, suggesting that PHR1(-like) control of transcriptional repression responses is indirect. In agreement with this, transcriptomic analysis of a transgenic plant expressing PHR1 fused to the hormone ligand domain of the glucocorticoid receptor showed that PHR1 direct targets (i.e., displaying altered expression after GR:PHR1 activation by dexamethasone in the presence of cycloheximide) corresponded largely to Pi starvation-induced genes that are highly enriched in P1BS. A minimal promoter containing a multimerised P1BS recapitulates Pi starvation-specific responsiveness. Likewise, mutation of P1BS in the promoter of two Pi starvation-responsive genes impaired their responsiveness to Pi starvation, but not to other stress types. Phylogenetic footprinting confirmed the importance of P1BS and PHR1 in Pi starvation responsiveness and indicated that P1BS acts in concert with other cis motifs. All together, our data show that PHR1 and PHL1 are partially redundant TF acting as central integrators of Pi starvation responses, both specific and generic. In addition, they indicate that transcriptional repression responses are an integral part of adaptive responses to stress.
Subfields: Plant Science
View Paper: https://www.semanticscholar.org/paper/f969c4143127b416a31d1dfadf06aadd78726820
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Citations in the corpus
- The Role of PHT1 Family Transporters in the Acquisition and Redistribution of Phosphorus in Plants (2019, background): “Nucleus localized PHR1 belongs to the MYB TF superfamily and binds to the P1BS or P1BS-like domain (GNATATNC) in PHT1 gene promoters and regulates the expression of PHT1 at LPC in Arabidopsis (Bustos et al., 2010), barley (Sch€unmann et al., 2004a; Sch€unmann et al., 2004b), rice (Meina et al., 2015”
- Plant abiotic stress response and nutrient use efficiency (2020, background): “For instance, it was reported that association with microbes can enhance the phosphate starvation response (PSR) in Arabidopsis by directly enhancing the activity of PHR1, the master regulator of the PSR response (Bustos et al., 2010; Rubio et al., 2001).”
- Signaling network in sensing phosphate availability in plants. (2011, background): “PHR1 and PHL1 bind to a DNA motif of GNATATNC, termed P1BS (10, 113).”
- Mechanism of Zinc absorption in plants: uptake, transport, translocation and accumulation (2016, background): “Regarding macronutrient interaction in regulation of Zn homeostasis, crosstalk between Zn and nitrogen (N) and between Zn and phosphorus (P) nutrition has been widely reported (Cakmak et al. 2010a, b; Bustos et al. 2010; Khan et al. 2014).”
- Root microbiota drive direct integration of phosphate stress and immunity (2017, neutral): “All these genes are expressed in roots 13–18 .”
- Phosphorus Plays Key Roles in Regulating Plants’ Physiological Responses to Abiotic Stresses (2023, background): “Following phosphorylation and subsequent translocation to the nucleus, transcription factors PHR1 and PHL1 play a pivotal role in orchestrating the activation of genes associated with phosphorus uptake, transport and utilization [91].”