Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps

By V. Papayannopoulos, Kathleen D. Metzler, Abdul Hakkim, Arturo Zychlinsky. Journal of Cell Biology, 2010

2,026 citations recorded by the source corpus.

Neutrophil elastase escapes azurophilic granules, translocates to the nucleus, and degrades histones to promote chromatin decondensation necessary for NET formation.

Subfields: Genetics, Immunology

View Paper: https://www.semanticscholar.org/paper/aa6dbaa50e29754413fe33dde425c6147f894d2b

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. Arturo Zychlinsky
2. V. Brinkmann
3. P. Kubes
4. Paul Martin
5. Oscar A. Peña
6. E. Wagner
7. Kathleen D. Metzler
8. V. Papayannopoulos

In Immunology

1. Guido Kroemer
2. P. Kubes
3. Arturo Zychlinsky
4. L. Galluzzi
5. V. Brinkmann
6. V. Papayannopoulos
7. N. Borregaard
8. L. Zitvogel

Citations in the corpus

• Cellular and molecular mechanisms of skin wound healing (2024, positive): “…is achieved by a combination of targeted ROS release and the formation of NETs (by release of their DNA into the extracellular space), in combination with antimicrobial molecules, in a process which is dependent on neutrophil elastase, peptidylarginine deaminase 4 (PAD4) and gasdermin D 86–89 (Fig.”
• Netting neutrophils induce endothelial damage, infiltrate tissues and expose immunostimulatory molecules in systemic lupus erythematosus (2011, background): “It has been shown that neutrophils from patients with anti-neutrophilic cytoplasmic Ab (ANCA)-positive vasculitis release NETs enriched in MPO and LL-37 (10).”
• Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018 (2018, background): “Moreover, ELANE is dispensable for NET formation in the course of deep vein thrombosis (in mice) [744].”
• Thrombosis: tangled up in NETs. (2014, background): “It was proposed that some neutrophil granular enzymes such as neutrophil elastase translocate to the nucleus and help in chromatin decondensation by cleavage of histones.(17) Genetic evidence determining to what extent individual granular proteins contribute to NETosis remains to be established.”
• A Myeloperoxidase-Containing Complex Regulates Neutrophil Elastase Release and Actin Dynamics during NETosis (2014, positive): “, 2007) and in a cell-free assay where neutrophil nuclei are incubated with cytoplasmic extracts containing azurophilic granules in vitro (Figure S2A; Papayannopoulos et al., 2010).”
• NETosis: how vital is it? (2013, background): “cleavage.(8) Myeloperoxidase (MPO) also contributes to the decondensation of nuclear DNA, although the mechanism is not evident because the process does not require the enzymatic function of MPO.”
• Diverse stimuli engage different neutrophil extracellular trap pathways (2017, background): “The hydrogen peroxide (H2O2) produced is in turn consumed by myeloperoxidase (MPO) to produce hypochlorous acid as well as other oxidants (Papayannopoulos et al., 2010).”
• Neutrophil Extracellular Traps and Its Implications in Inflammation: An Overview (2017, background): “NET formation is dependent on elastase and myeloperoxidase transport all the way from granules to the nucleus (18).”