A light-inducible CRISPR/Cas9 system for control of endogenous gene activation

By Lauren R. Polstein, C. Gersbach. Nature Chemical Biology, 2015

606 citations recorded by the source corpus.

Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.

Subfields: Cellular and Molecular Neuroscience, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/a9263e14fcfc7bc4faf29b27f64d4c6636952797

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cellular and Molecular Neuroscience

1. Jerilyn A. Timlin
2. J. Carney
3. J. C. Harper
4. Christopher A. Lino

In Molecular Biology

1. Haifeng Wang
2. Marie La Russa
3. Jerilyn A. Timlin
4. J. Carney
5. J. C. Harper
6. Lei S. Qi
7. Christopher A. Lino

In Plant Science

1. Haifeng Wang
2. Jerilyn A. Timlin
3. J. Carney
4. J. C. Harper
5. Lei S. Qi
6. Marie La Russa
7. Christopher A. Lino

Citations in the corpus

• CRISPR/Cas9 in Genome Editing and Beyond. (2016, background): “(231) and Polstein & Gersbach (232) developed light-inducible CRISPRa systems to activate endogenous gene expression when cells are exposed to blue light, thus allowing rapid and reversible control of gene expression.”
• Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering. (2016, neutral): “Recent work has focused on developing various chemical- and light-inducible Cas9 constructs to allow for greater spatiotemporal control and on employing Cas9 orthologs with different PAM sequences and smaller sizes, allowing for easier packaging in adeno-associated virus vectors (Davis et al., 2015;”
• Delivering CRISPR: a review of the challenges and approaches (2018, methodology): “A similar blue light activating system was developed for the epigenetic gene activator CRISPR system of Hilton et al. discussed above (Polstein & Gersbach, 2015).”