Environmental DNA for wildlife biology and biodiversity monitoring.

By Kristine Bohmann, Alice Evans, M. T. P. Gilbert, Gary R. Carvalho, S. Creer, M. Knapp, Douglas W. Yu, M. de Bruyn. Trends in Ecology & Evolution, 2014

1,225 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a9067ce528f3982ea03d9bd7c463ce65f3966d94

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Citations in the corpus

A comprehensive and comparative evaluation of primers for metabarcoding eDNA from fish (2020, background): “Environmental DNA (eDNA)-based species detection has revolutionised the manner in which biodiversity is surveyed and has proven to be a highly effective, efficient, economical and non-invasive approach to biomonitoring (Bohmann et al., 2014; Deiner et al., 2017; Taberlet et al., 2012).”
Long‐range PCR allows sequencing of mitochondrial genomes from environmental DNA (2017, result): “These results contradict the common assumption that eDNA from communities of living fishes in water is highly degraded (e.g. Bohmann et al., 2014).”
Protecting an ecosystem service: approaches to understanding and mitigating threats to wild insect pollinators (2016, background): “…chloroplast genome sequencing is expanding rapidly, which will overcome the 790 problem of low chloroplast abundance in the pollen, currently addressed with sequencing of 791 the nuclear ITS region (Bohmann et al., 2014; Keller et al., 2015; Richardson et al., 2015; 792 Sickel et al., 2015).”
Applications of next-generation sequencing to the study of biological invasions (2015, methodology): “…conservation biology (Angeloni et al., 2012), microbial metagenomics (Laserson et al., 2011), environmental monitoring (Ficetola et al., 2008; Bohmann et al., 2014) and crop genetics Sequencing costs (note log-scale axis) were obtained from the National Human Genome Research Institute…”
Taxonomic and geographical representation of freshwater environmental DNA research in aquatic conservation (2019, background): “Environmental DNA (eDNA) techniques are gaining increasing importance in ecological and conservation sciences (Bohmann et al., 2014; Corlett, 2017; Lawson Handley, 2015; Leese et al., 2016; Rees, Maddison, Middleditch, Patmore, & Gough, 2014; Thomsen & Willerslev, 2015).”
Connected macroalgal‐sediment systems: blue carbon and food webs in the deep coastal ocean (2019, methodology): “During a 13-month research program, we combined environmental DNA (eDNA) sequencing (Bohmann et al. 2014) and Bayesian Mixing Modeling of bulk stable isotope data (Phillips et al. 2014) as complementary biotracing techniques (Nielsen et al. 2018) with direct Article e01366; page 2 ANAMOURAQUEIR OS…”
Reconstructing hyperdiverse food webs: Gut content metabarcoding as a tool to disentangle trophic interactions on coral reefs (2019, background): “However, there remain certain groups of organisms (e.g. meiofauna that are difficult to visually detect) that are under‐represented by metabarcoding data simply due to the absence of barcoding data (Bohmann et al., 2014).”
Can DNA-Based Ecosystem Assessments Quantify Species Abundance? Testing Primer Bias and Biomass—Sequence Relationships with an Innovative Metabarcoding Protocol (2015, background): “Metabarcoding is currently being tested to address a wide range of biological problems, such as invasive species detection [28], gut content analysis [29], and assessment of microbial [30] and metazoan diversity, such as that of arthropods (e.”
Diet tracing in ecology: Method comparison and selection (2017, background): “Both approaches are similar to environmental analyses (eDNA is reviewed by Bohmann et al., 2014), though inhibitors (DNA preservation, extraction, amplification) in the gut may present problems (Schrader, Schielke, Ellerbroek, & Johne, 2012).”
Integrating dispersal proxies in ecological and environmental research in the freshwater realm (2017, background): “These include 207 population genetics (Hughes, 2007), DNA-barcoding (Cristescu 2014) and environmental 208 Page 10 of 70 https://mc06.manuscriptcentral.com/er-pubs Environmental Reviews Draft 11 DNA (Bohmann et al. 2014).”
Assessing different components of diversity across a river network using eDNA (2019, background): “…of high‐throughput sequencing, which allowed not only detection of single species but also detection of whole communi‐ ties, eDNA metabarcoding has been proposed to revolutionize biodiversity assessments (Bohmann et al., 2014; Lawson Handley, 2015; Shokralla, Spall, Gibson, & Hajibabaei, 2012).”
Downstream transport and seasonal variation in freshwater pearl mussel ( Margaritifera margaritifera ) eDNA concentration (2019, methodology): “This method has mainly been applied to detect rare and threatened species, invasive species and species that are difficult to monitor with conventional methods (Bohmann et al., 2014; Jones, 2013).”
Complex patterns of global spread in invasive insects: eco-evolutionary and management consequences (2016, background): “Prospects for the future Molecular tools have powerful potential for detecting the spread of pests and pathogens, but there are many challenges (Armstrong and Ball 2005; Bohmann et al. 2014; Chown et al. 2014; Lawson Handley 2015).”
Selecting cost effective and policy-relevant biological indicators for European monitoring of soil biodiversity and ecosystem function (2016, background): “The abundance of 523 functional genes was normalized (gene copy number per ng of DNA), which could explain why some 524 were less discriminatory.”