Dispersion and degradation of environmental DNA from caged fish in a marine environment

By Hiroaki Murakami, Seokjin Yoon, A. Kasai, Toshifumi Minamoto, Satoshi Yamamoto, Masayuki K. Sakata, Tomoya Horiuchi, Hideki Sawada, Michio Kondoh, Y. Yamashita and 1 more. Fisheries Science, 2019

134 citations recorded by the source corpus.

Environmental DNA (eDNA) consists of DNA fragments shed from organisms into the environment, and can be used to identify species presence and abundance. This study aimed to reveal the dispersion and degradation processes of eDNA in the sea. Caged fish were set off the end of a pier in Maizuru Bay, the Sea of Japan, and their eDNA was traced at sampling stations located at the cage and 10, 30, 100, 300, 600 and 1000 m distances from the cage along two transect lines. Sea surface water was collected at each station at 0, 2, 4, 8, 24 and 48 h after setting the cage, and again after removing the cage. Quantitative PCR analyses using a species-specific primer and probe set revealed that the target DNA was detectable while the cage was present and for up to 1 h after removing the cage, but not at 2 h or later. Among the 57 amplified samples, 45 (79%) were collected within 30 m from the cage. These results suggest that eDNA can provide a snapshot of organisms present in a coastal marine environment.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a8fd27407923d6fdfabeada38fab4ec5d890d898

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Citations in the corpus

Environmental DNA (eDNA) as a tool for assessing fish biomass: A review of approaches and future considerations for resource surveys (2021, background): “Similarly, most detections of striped jack (Pseudocaranx dentex) were within 30 m of holding cages at the same study location, with no effect of flow direction on eDNA detections, although eDNA concentrations did decrease with distance from the cages (Murakami et al., 2019) (Table 2).”
Towards the Optimization of eDNA/eRNA Sampling Technologies for Marine Biosecurity Surveillance (2021, background): “However, the influence of varying abiotic factors on the persistence of eDNA complicates the sampling design and interpretation of results, e.g., in the context of the current presence and affinity of a viable population of target (pest) species [57–59].”
Sedimentary eDNA provides different information on timescale and fish species composition compared with aqueous eDNA (2020, background): “Furthermore, basic properties of eDNA, such as release and decay rates and transportation distance in natural water bodies, have also been investigated (Barnes & Turner, 2016; Jane et al., 2015; Jo et al., 2017; Murakami et al., 2019).”
Trawl and eDNA assessment of marine fish diversity, seasonality, and relative abundance in coastal New Jersey, USA (2021, background): “, 2017), within 100 m and 1 h of removal of caged striped jack in a marine environment (Murakami et al., 2019), and between samples collected immediately above and below a halocline (Jeunen et al.”
MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples (2020, background): “2019; Beng and Corlett 2020), it is essential to consider the effects of environmental factors on those processes for a more appropriate interpretation of eDNA detections (Yamamoto et al. 2016; Sassoubre et al. 2016; Murakami et al. 2019).”