Direct observation of individual endogenous protein complexes in situ by proximity ligation

By O. Söderberg, M. Gullberg, Malin Jarvius, Karin K. Ridderstråle, Karl-Johan Leuchowius, Jonas Jarvius, K. Wester, P. Hydbring, F. Bahram, L. Larsson and 1 more. Nature Methods, 2006

2,445 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a8b6a9ab6df4552b09873ec29218f729ee6aa05f

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In Molecular Biology

1. Kevin J. Tracey
2. V. Deretic
3. M. Ochani
4. U. Andersson
5. M. Rosas‐Ballina
6. T. Johansen
7. Shizuo Akira
8. R. Medzhitov

Citations in the corpus

• TBK-1 promotes autophagy-mediated antimicrobial defense by controlling autophagosome maturation (2012, positive): “Procedures used for proximity ligation in situ assay (P-LISA) (Söderberg et al., 2006) were in strict accordance with the manufacturer’s instructions (Olink Bioscience, Uppsala, Sweden).”
• A critical cysteine is required for HMGB1 binding to Toll-like receptor 4 and activation of macrophage cytokine release (2010, methodology): “To investigate whether endogenous HMGB1 has the capability to interact with TLR4 we used in situ proximity ligation assay (PLA), which is a unique method developed to visualize subcellular localization and protein-protein interactions in situ (22).”