Overexpression of the Arabidopsis H+-PPase enhanced resistance to salt and drought stress in transgenic alfalfa (Medicago sativa L.).

By A. Bao, Suo-min Wang, Guo-Qiang Wu, Jiejun Xi, Jinlin Zhang, Chun-Mei Wang. 2009

264 citations recorded by the source corpus.

Abstract Salinity and drought are two major abiotic factors limiting crop production. To generate the legume forage adapting to saline and arid soils, we had transformed alfalfa ( Medicago sativa ) with AVP1 , a vacuolar H + -pyrophosphatase (H + -PPase) gene from Arabidopsis thaliana . In this paper, we report that overexpression of the AVP1 gene confers enhanced salt and drought tolerance to the transformed alfalfa. Transgenic alfalfa grows well in the presence of 200 mM NaCl and also under a water-deprivation condition, while wild-type plants exhibit chlorosis and growth inhibition, even death. Compared with wild-type plants, transgenic plants accumulate more Na + , K + and Ca 2+ in leaves and roots. Moreover, the leaves of transgenic plants retain more water during drought stress than those of wild-type plants due to lower solute potential. Increased solute accumulation and water retention, and steady intracellular ion homeostasis might also confer other phenotypes of salt and drought tolerance in the transgenic plants, which include the higher photosynthesis capacity and the lesser cell membrane damage during salt or water-deficit stress. Furthermore, the increased potassium uptake and root activity in transgenic alfalfa may be the consequences of rhizosphere acidification resulting from expression of the AVP1 . These results indicated that the expression of AVP1 confers enhanced salt and drought tolerance on alfalfa, a very important crop. This study provides a way for improving salt and drought tolerance in important legume forages.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/a850274977830ffc615aa47907b414a95090d0eb

How researchers see this paper

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In Molecular Biology

1. T. Coba de la Peña
2. Mohamed Magdy F. Mansour
3. J. Pueyo

In Plant Science

1. T. Coba de la Peña
2. Hui Chen
3. Mohamed Magdy F. Mansour
4. J. Pueyo
5. Jian-Guo Jiang

Citations in the corpus

• Osmotic adjustment and plant adaptation to environmental changes related to drought and salinity. (2010, background): “), drought and salinity are considered the main factors limiting plant growth and yield in many areas (Bao et al. 2009; Lee and Hwang 2009).”
• Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, unknown): “Metal concentrations were measured by using inductively coupled plasma optical emission spectrometry SPS3000 (Seiko, Tokyo, Japan).”
• Plasma membrane permeability as an indicator of salt tolerance in plants (2013, background): “2006) and transgenic alfalfa (Bao et al. 2009) overexpressing the H-pyrophosphatase (H-PPase) than in the respective wild type under salt stress.”
• Legumes in the reclamation of marginal soils, from cultivar and inoculant selection to transgenic approaches (2011, background): “Alfalfa has been transformed with the AVP1 gene, a vacuolar H+-pyrophosphatase (H+-PPase) from Arabidopsis thaliana (Bao et al. 2009).”