Multifunctional in vivo vascular imaging using near-infrared II fluorescence

By Guosong Hong, Jerry C. Lee, J. Robinson, U. Raaz, Liming Xie, N. Huang, J. Cooke, Hongjie Dai. Nature Medicine, 2012

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In vivo real-time epifluorescence imaging of mouse hind limb vasculatures in the second near-infrared region (NIR-II) is performed using single-walled carbon nanotubes as fluorophores. Both high spatial (∼30 μm) and temporal (<200 ms per frame) resolution for small-vessel imaging are achieved at 1–3 mm deep in the hind limb owing to the beneficial NIR-II optical window that affords deep anatomical penetration and low scattering. This spatial resolution is unattainable by traditional NIR imaging (NIR-I) or microscopic computed tomography, and the temporal resolution far exceeds scanning microscopic imaging techniques. Arterial and venous vessels are unambiguously differentiated using a dynamic contrast-enhanced NIR-II imaging technique on the basis of their distinct hemodynamics. Further, the deep tissue penetration and high spatial and temporal resolution of NIR-II imaging allow for precise quantifications of blood velocity in both normal and ischemic femoral arteries, which are beyond the capabilities of ultrasonography at lower blood velocities.

Subfields: Biophysics, Radiology, Nuclear Medicine and Imaging, Surgery

View Paper: https://www.semanticscholar.org/paper/a761bf91c5df9960d02787ba93c28e5618c73481

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In Radiology, Nuclear Medicine and Imaging

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Through-skull fluorescence imaging of the brain in a new near-infrared window (2014, background): “22 In the healthy Mouse C1, the linearly rising fluorescence signals in both cerebral hemispheres had almost identical slopes, suggesting a relative perfusion of ~1 (see SI for perfusion analysis) (Fig.”