Through-skull fluorescence imaging of the brain in a new near-infrared window
By Guosong Hong, Shuo Diao, Junlei Chang, A. Antaris, Changxin Chen, Bo Zhang, Su Zhao, D. Atochin, Paul L Huang, K. Andreasson and 2 more. Nature Photonics, 2014
933 citations recorded by the source corpus.
To date, brain imaging has largely relied on X-ray computed tomography and magnetic resonance angiography, with their limited spatial resolution and long scanning times. Fluorescence-based brain imaging in the visible and traditional near-infrared regions (400–900 nm) is an alternative, but at present it requires craniotomy, cranial windows and skull-thinning techniques, and the penetration depth is limited to 1–2 mm due to light scattering. Here, we report through-scalp and through-skull fluorescence imaging of mouse cerebral vasculature without craniotomy, utilizing the intrinsic photoluminescence of single-walled carbon nanotubes in the 1.3–1.4 μm near-infrared window (NIR-IIa window). Reduced photon scattering in this spectral region allows fluorescence imaging to a depth of >2 mm in mouse brain with sub-10-μm resolution. An imaging rate of ∼5.3 frames per second allows for dynamic recording of blood perfusion in the cerebral vessels with sufficient temporal resolution, providing real-time assessment of a blood flow anomaly in a mouse middle cerebral artery occlusion stroke model. Near-infrared photoluminescence from carbon nanotubes makes it possible to optically image the vasculature in the brain directly through the skull.
Subfields: Biomedical Engineering, Radiology, Nuclear Medicine and Imaging
View Paper: https://www.semanticscholar.org/paper/0d942b17b8ffc2124447dc91111b8018edfadd48
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In Biomedical Engineering
- A. Antaris
- Guosong Hong
- Shuo Diao
- Hongjie Dai
- Zhuang Liu
- Kevin D. Welsher
- Yun Wang
- John Tze-Wei Yeow
In Radiology, Nuclear Medicine and Imaging
Citations in the corpus
- Clarifying Tissue Clearing (2015, neutral): “4 mm has recently been identified and used to image >2 mm into tissue, even passing through bone (Hong et al., 2014).”
- Biological imaging without autofluorescence in the second near-infrared region (2015, methodology): “We first measured autofluorescence from mouse organs in the entire NIR-II (1,000–1,700 nm), NIR-IIb (1,500– 1,700 nm), and the previously defined NIR-IIa (1,300– 1,400 nm) [16] regions using a 2D InGaAs camera (Princeton Instruments, response up to 1,700 nm.”