Recombineering: a powerful new tool for mouse functional genomics

By Neal G. Copeland, Nancy A. Jenkins, D. Court. Nature reviews genetics, 2001

787 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/a70689945ffa2db9ba07c391078c1db067f8d310

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Citations in the corpus

Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli. (2006, positive): “2 (2006) the method may increase the capacities for the production of transgenic animals (7) without the introduction of unnecessary scars, such as loxP sites, within modified sequences (23).”
miR-146a is a significant brake on autoimmunity, myeloproliferation, and cancer in mice (2011, methodology): “The assembly of the targeting construct was performed by recombineering in bacteria according to the protocol established by Copeland et al. (2001).”
A highly efficient recombineering-based method for generating conditional knockout mutations. (2003, background): “This new form of chromosome engineering, termed recombineering (Copeland et al. 2001; Muyrers et al. 2001), makes it possible to introduce loxP sites and selectable markers anywhere in a gene, and greatly shortens the time it takes to make a targeting vector.”
Embryonic stem cells: prospects for developmental biology and cell therapy. (2005, background): “Recent innovations use homologous recombination to construct targeting vectors in a process termed recombineering (85, 242).”
A gene expression atlas of the central nervous system based on bacterial artificial chromosomes (2003, methodology): “The basic strategy for the project is to: (1) select genes to be analysed; (2) prepare modified BACs in which an EGFP reporter gene is substituted for the gene of interest; (3) prepare transgenic lines carrying each reporter construct; (4) analyse systematically reporter gene expression in these lin”
Simple and highly efficient BAC recombineering using galK selection (2005, methodology): “Recombineering (recombination-mediated genetic engineering) makes it possible to modify BAC DNA via homologous recombination [reviewed in (6,7)].”
Immunogenicity of induced pluripotent stem cells (2011, positive): “To increase the frequency of homologous recombination in hESCs, we employed the recombineering technology to develop bacterial artificial chromosome (BAC)-based targeting vectors that harbor much larger size of homologous arms (Copeland et al., 2001).”