Sugars and Circadian Regulation Make Major Contributions to the Global Regulation of Diurnal Gene Expression in Arabidopsis[W][OA]

By O. Bläsing, Y. Gibon, Manuela Günther, Melanie Höhne, R. Morcuende, D. Osuna, O. Thimm, Björn Usadel, W. Scheible, Mark Stitt. The Plant Cell Online, 2005

708 citations recorded by the source corpus.

The diurnal cycle strongly influences many plant metabolic and physiological processes. Arabidopsis thaliana rosettes were harvested six times during 12-h-light/12-h-dark treatments to investigate changes in gene expression using ATH1 arrays. Diagnostic gene sets were identified from published or in-house expression profiles of the response to light, sugar, nitrogen, and water deficit in seedlings and 4 h of darkness or illumination at ambient or compensation point [CO2]. Many sugar-responsive genes showed large diurnal expression changes, whose timing matched that of the diurnal changes of sugars. A set of circadian-regulated genes also showed large diurnal changes in expression. Comparison of published results from a free-running cycle with the diurnal changes in Columbia-0 (Col-0) and the starchless phosphoglucomutase (pgm) mutant indicated that sugars modify the expression of up to half of the clock-regulated genes. Principle component analysis identified genes that make large contributions to diurnal changes and confirmed that sugar and circadian regulation are the major inputs in Col-0 but that sugars dominate the response in pgm. Most of the changes in pgm are triggered by low sugar levels during the night rather than high levels in the light, highlighting the importance of responses to low sugar in diurnal gene regulation. We identified a set of candidate regulatory genes that show robust responses to alterations in sugar levels and change markedly during the diurnal cycle.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/a68929b431da75a4846c41ea979c47d18231a027

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Citations in the corpus

Regulatory roles of sugars in plant growth and development (2018, methodology): “The sugars accumulated under drought or cold mainly play the role of osmoregulators and cryoprotectans, but also as a stored source of carbon for later use (when the stress factor ceases to exist); they can also act as regulators modulating plant growth under new conditions [10,12,15,17,18,69].”
Sugar sensing and signaling in plants: conserved and novel mechanisms. (2006, background): “Energyconsuming biosynthetic processes, including protein synthesis, are switched off (11, 23, 58, 154).”
PageMan: An interactive ontology tool to generate, display, and annotate overview graphs for profiling experiments (2006, background): “There is now a trend to more complex experiments, consisting of time-course (e.g. [4,5]) or dose-response studies.”
Effects of nitrogen deficiency on leaf photosynthesis, carbohydrate status and biomass production in two olive cultivars 'Meski' and 'Koroneiki' (2010, background): “So, the reduction of photosynthesis in N-deficient olive plants is also a direct consequence of sugar next to starch accumulation, because they both exert metabolite feedback regulation (Koch, 2004; Bläsing et al., 2005).”
Potent Induction of Arabidopsis thaliana Flowering by Elevated Growth Temperature (2006, neutral): “Therefore, we visually inspected the genes that show an altered profile during light shift for their behavior in a diurnal dataset [56].”