Culture of Preimplantation Mouse Embryos Affects Fetal Development and the Expression of Imprinted Genes1

By S. Khosla, W. Dean, David Brown, Wolf Reik, R. Feil. Biology of Reproduction, 2001

612 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology, Pediatrics, Perinatology and Child Health

View Paper: https://www.semanticscholar.org/paper/a4ca2e6c76998e85b3f3d69a6df8614b7b03f657

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. Andrew P. Feinberg
2. Keith D. Robertson
3. M. Fraga
4. R. Feil
5. Felix Krueger
6. Simon R. Andrews
7. Manel Esteller
8. S. Baylin

In Molecular Biology

1. Keith D. Robertson
2. M. Fraga
3. Andrew P. Feinberg
4. R. Feil
5. Manel Esteller
6. S. Baylin
7. Felix Krueger
8. Simon R. Andrews

In Pediatrics, Perinatology and Child Health

1. Andrew P. Feinberg
2. Keith D. Robertson

Citations in the corpus

• Epigenetics and the environment: emerging patterns and implications (2012, background): “Indeed, altered DNA methylation patterns that are acquired during early embryonic development are maintained throughout later development at imprinted genes and at some other loc”
• DNA methylation and human disease (2005, background): “Importantly, imprinted DNA methylation patterns are frequently disrupted by in vitro manipulation of embryos in both animals and human”
• Birth of Piglets Derived from Porcine Zygotes Cultured in a Chemically Defined Medium1 (2002, background): “Whereas we demonstrated that porcine zygotes cultured for 4 days in a chemically defined medium showed full-term developmental potential without any severe reduction in embryo viability, recent studies have shown that the preimplantation environment affects subsequent fetal and postnatal growth [45,”
• Child health, developmental plasticity, and epigenetic programming. (2011, background): “This in vitro culturing step frequently leads to altered DNA methylation at ICRs and hence, perturbed imprinted gene expression and phenotype when fetal calf serum is added to the culture medium (278, 285, 300).”
• Association of in vitro fertilization with Beckwith-Wiedemann syndrome and epigenetic alterations of LIT1 and H19. (2003, background): “…preimplantation mouse embryos cultured in a chemically defined medium (M16) undergo epigenetic alterations in the H19, IGF2, Grb10, and Grb7 genes (Khosla et al. 2001); mouse embryos cultured in Whitten’s medium can undergo LOI of H19 (Doherty et al. 2000); and cloned mice show variations in…”