Direct analysis of wood-inhabiting fungi using denaturing gradient gel electrophoresis of amplified ribosomal DNA

By E. Vainio, J. Hantula. 2000

627 citations recorded by the source corpus.

Subfields: Cell Biology, Insect Science, Plant Science

View Paper: https://www.semanticscholar.org/paper/a44edf4e7df9144833eb7ba59dda44a55809c94a

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cell Biology

In Insect Science

In Plant Science

Citations in the corpus

Microbial diversity in soil: ecological theories, the contribution of molecular techniques and the impact of transgenic plants and transgenic microorganisms (2004, neutral): “developed by Vainio and Hantula (2000) have been”
Ammonia-oxidizing bacteria: a model for molecular microbial ecology. (2001, background): “Such limitations may be partially circumvented by the use of inosine residues at appropriate degenerate primer positions (185).”
Indoor wood-decay basidiomycetes: damage, causal fungi, physiology, identification and characterization, prevention and control (2007, background): “Most investigations on wood-decay fungi use the PCR (e.g. Vainio and Hantula 2000; Hietala et al. 2003; Eikenes et al. 2005).”
Biological Soil Disinfestation (BSD), a new control method for potato brown rot, caused by Ralstonia solanacearum race 3 biovar 2 (2007, methodology): “The PCR used the U968 (40 bp GC clamp) and L1401 universal eubacterial primers (Heuer & Smalla, 1997) and primers FR1 (58 bp BC-clamp) and FF390 ( Vainio & Hantula, 2000 ) for amplification of fungal smallsubunit (SSU) rDNA.”
Soil nitrogen cycling rates in low arctic shrub tundra are enhanced by litter feedbacks (2010, methodology): “…for the amplification of eubacterial 16S rRNA gene (Chu et al. 2007) and the primer pair FF390 (forward) and FR1-GC (reverse) was used for the amplification of the fungal 18S rRNA gene (Vainio and Hantula 2000) with a nested PCR (FF390 and FR1 for the 1st PCR, FF390 and FR1-GC for the 2nd PCR).”
Comparison of 18S rDNA primers for estimating fungal diversity in agricultural soils using polymerase chain reaction-denaturing gradient gel electrophoresis (2008, methodology): “Vainio and Hantula (2000) also reported low diversity because of short fragments being amplified with primer set FF390/FR1GC compared with NS1/FR1-GC, which produces long fragments covering almost the entire 18S rDNA sequence.”
Microbial composition and diversity of an upland red soil under long-term fertilization treatments as revealed by culture-dependent and culture-independent approaches (2008, background): “Vainio and Hantula (2000) showed that DGGE detected more fungal species than the culturing method from environment samples.”
The Influence of Vegetation Type on the Dominant Soil Bacteria, Archaea, and Fungi in a Low Arctic Tundra Landscape (2011, methodology): “Fungal 18S rRNA gene sequences were amplifi ed using primer pairs FF390 (5′-CGATAACGAACGAGACCT-3′) and FR1 (5′AICCATTCAATCGGTAIT-3′), and re-amplifi ed with FF390 and FR1-GC (Vainio and Hantula, 2000).”
The role of terrestrially derived organic carbon in the coastal ocean: A changing paradigm and the priming effect (2011, background): “Because not all bacteria found in wood are actually wooddecaying species, in general, the rRNA diversity is typically less than 20 taxa, particularly in samples from marine sites (27).”
DGGE coupled with ribosomal DNA gene phylogenies reveal uncharacterized fungal phylotypes (2006, neutral): “Vainio and Hantula (2000) studied wood-inhabiting fungi and found that phylotypes isolated from environmental samples were comparable to fungi isolated from the same substrate at varying depths.”