Extraction of DNA from soil

By P. Robe, R. Nalin, Carmela Capellano, T. Vogel, P. Simonet. 2003

328 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/a44ad597977f8575ad6f4003aedd637e00946325

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In Ecology

In Molecular Biology

Citations in the corpus

Inoculation of wheat with Azospirillum brasilense and Pseudomonas fluorescens: Impact on the production and culturable rhizosphere microflora (2009, unknown): “ture use and environmental protection [43].”
Molecular approaches to study the insect gut symbiotic microbiota at the ‘omics’ age (2010, background): “, 1991) or by gradient centrifugation in media such as sucrose, Nycodenz R ©, Percoll R © or metrizamide (Pillai et al., 1991; Robe et al., 2003).”
Extracellular DNA in soil and sediment: fate and ecological relevance (2009, methodology): “Despite the existence of several methods for the extraction of iDNA from soil (Robe et al. 2003; Bakken and Frostegård 2006; Whitehous and Hannah 2007; Lakay et al. 2007), a few methods have been proposed to extract eDNA from soils and sediments; the latter ones are usually based on the absence of…”
Thematic Issue on Horizontal Gene Transfer Review Release and persistence of extracellular DNA in the environment (2007, unknown): “For instance, the level of DNA recovery from soil ﬂuctuates between 8–99% (Robe et al., 2003; Zhou et al., 1996).”
Soil biodiversity and DNA barcodes: opportunities and challenges (2015, methodology): “There is a high variability in all steps of the analysis: from the sampling strategy to the DNA extraction procedure (Robe et al., 2003), sequencing method (i.e. different NGS-platforms) and tools and parameters for data analysis (e.g. choice of bioinformatics software and genetic distance…”
Molecular tools in rhizosphere microbiology—from single-cell to whole-community analysis (2009, methodology): “Approaches combining centrifugation-based cell separation from soil particles and in-plug lysis and pulsed field gel electrophoresis (PFGE) after bacteria are embedded in the agarose plugs are now recognized as the most efficient method yielding DNA fragments more than 300 kbp in size and providing ”
Culture-independent molecular techniques for soil microbial ecology (2010, methodology): “Other methods of cell lysis include sonication, grindingefreezinge thawing, solubilisation of cell walls and membranes by detergents or degradation of these structures by boiling and/or enzymatic means (Robe et al., 2003).”