Terminal Restriction Fragment Length Polymorphism Analysis Program, a Web-Based Research Tool for Microbial Community Analysis

By T. Marsh, P. Saxman, J. Cole, J. Tiedje. Applied and Environmental Microbiology, 2000

330 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/a3f6b40797d9cbf70cba253a1c66d062a30a4aa8

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Citations in the corpus

Rumen Microbial Population Dynamics during Adaptation to a High-Grain Diet (2010, methodology): “T-RFLP analysis (22, 27, 32) was performed using 100 ng of”
Microbial diversity in soil: ecological theories, the contribution of molecular techniques and the impact of transgenic plants and transgenic microorganisms (2004, methodology): “T-RFLP analysis is based on the restriction endonuclease digestion of fluorescent end-labelled PCR amplicons (Avaniss-Aghajani 1994; Liu et al. 1997; Marsh et al. 2000; Osborn et al. 2000).”
T-REX: software for the processing and analysis of T-RFLP data (2009, methodology): “Most software that has been developed is aimed at referencing T-RFLP profiles with a sequence database (e.g. TAP-TRFLP [11,12], MiCA [13], PAT [14], TRAMPR [15].”
Phylogenetic diversity of dissimilatory ferric iron reducers in paddy soil of Hunan, South China (2009, methodology): “In addition, the T-RFLP technique is inherently biased for underestimating the diversity in a given sample owing to the conservation of enzyme recognition site sequences in a given gene across different organism (Marsh et al. 2000).”
The RDP-II (Ribosomal Database Project) (2001, background): “0 of the terminal restriction fragment polymorphism (T-RFLP) program (8) is under development.”
Effects of mulching and fertilization on soil nutrients, microbial activity and rhizosphere bacterial community structure determined by analysis of TRFLPs of PCR-amplified 16S rRNA genes (2002, methodology): “The TRF lengths of dominant culturable fluorescent pseudomonads in the rhizosphere samples from bare soil (B− and B+) and wood mulched (W− and W+) plots (Table 7) did not correspond to those predicted for fluorescent Pseudomonas strains using the TRFLP-TAP tool of in the 16S rRNA gene sequence datab”
Application of Denaturing Gradient Gel Electrophoresis (DGGE) To Study the Diversity of Marine Picoeukaryotic Assemblages and Comparison of DGGE with Other Molecular Techniques (2001, background): “This has been pointed out previously for prokaryotes (29, 31).”
Response of soil microbial communities to compost amendments (2006, methodology): “…in R. solani damping-off observed for the soil of Dijon amended with F SMC, we attempted to identify them by comparing their length with TRF sizes predicted for known microorganisms, using the T-RFLP analysis program (TAP) tool of the Ribosomal Database Project (RDP) web site (Marsh et al., 2000).”