Principles of two-photon excitation microscopy and its applications to neuroscience.

By Karel Svoboda, R. Yasuda. Neuron, 2006

1,014 citations recorded by the source corpus.

Subfields: Biophysics, Cellular and Molecular Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a340bbab392db5b78fc67b5741730dd56af8e30e

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Citations in the corpus

Rapid Redistribution of Synaptic PSD-95 in the Neocortex In Vivo (2006, methodology): “We used dual laser two-photon microscopy [56,62] and twophoton photoactivation of paGFP [52] to study the dynamics of PSD-95 in L2/3 dendrites in vivo.”
Multiple-Color Optical Activation, Silencing, and Desynchronization of Neural Activity, with Single-Spike Temporal Resolution (2007, background): “Two-photon excitation methods will likely prove valuable for driving excitatory and inhibitory opsin activity in neurons up to 1 mm deep [57].”
Brain-Machine Interfaces: From Basic Science to Neuroprostheses and Neurorehabilitation. (2017, methodology): “A particularly powerful method, two-photon excitation laser scanning microscopy, allows minimally invasive, three-dimensional sampling with submicrometer resolution (353, 591, 782).”
An optical neural interface: in vivo control of rodent motor cortex with integrated fiberoptic and optogenetic technology (2007, background): “perhaps deeper into cortex using two-photon systems, surface imaging systems based on microscope objectives could be adapted to deliver light through window craniotomies [45].”
Scaling Brain Size, Keeping Timing: Evolutionary Preservation of Brain Rhythms (2013, positive): “, 2012), complemented with high spatial-resolution imaging (Svoboda and Yasuda, 2006) and computational modeling (Wang, 2010) to aid in our understanding of the mechanisms and utility of oscillations.”
AI in Medical Imaging Informatics: Current Challenges and Future Directions (2020, neutral): “Two-photon ﬂuorescence imaging uses two photons of similar frequencies to excite molecules which allows for deeper penetration of tissue and lower phototoxicity (damage to living tissue caused by the excitation source) [33].”
Experience-dependent structural synaptic plasticity in the mammalian brain (2009, methodology): “html">Supplementary information S1 (table) for a summary of results) that used two-photon excitation laser scanning microscop”
Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window (2009, neutral): “(2) Spines often arise from thickenings in the dendritic shaft.”
Genetic dissection of neural circuits. (2008, positive): “The confluence of advances in genetically encoded indicators, deep-tissuemicroscopy (reviewed in Flusberg et al., 2005; Helmchen and Denk, 2005; Svoboda and Yasuda, 2006), and genetic”