Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing

By R. Tice, E. Agurell, Diana Anderson, B. Burlinson, A. Hartmann, H. Kobayashi, Y. Miyamae, E. Rojas, J. Ryu, Y. Sasaki. Environmental and Molecular Mutagenesis, 2000

4,966 citations recorded by the source corpus.

Subfields: Cancer Research, Health, Toxicology and Mutagenesis, Plant Science

View Paper: https://www.semanticscholar.org/paper/a288013876081528ded140139b1b1a7d4e22b11e

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In Cancer Research

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Citations in the corpus

• Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips (2006, neutral): “The single cell gel electrophoresis (SCGE) assay, commonly called the comet assay, is a genotoxicity test able to detect DNA damage induced by alkylating, intercalating and oxidizing agents (Tice et al., 2000; KoszVnenchak and Rokosz, 1997).”
• The comet assay for DNA damage and repair (2004, background): “Genotoxicity is also assessed in cell culture systems, on their own or in conjunction with the microsomal “S9” fraction from liver, that provides enzymes to metabolize chemicals to more reactive forms ( 26 ).”
• Zinc oxide nanoparticles induce oxidative DNA damage and ROS-triggered mitochondria mediated apoptosis in human liver cells (HepG2) (2012, methodology): “Images from 50 random cells (25 from each replicate slide) were analyzed for each experiment as per the guidelines [29].”
• NanoGenotoxicology: the DNA damaging potential of engineered nanomaterials. (2009, background): “Analysis of the length and fluorescence intensity of the comet tail is directly proportional to the amount of DNA damage [196,197].”
• CHO cell cytotoxicity and genotoxicity analyses of disinfection by-products: An updated review. (2017, background): “The SCGE or Comet assay is a molecular genetic assay that can quantitatively measure the level of genomic DNA damage induced in individual nuclei of cells (Fairbairn et al., 1995; Rundell et al., 2003; Tice et al., 2000).”
• Recommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop. (2003, methodology): “In the present recommendations, minimal standards for obtaining reproducible and reliable Comet assay data were identified which slightly deviate from those concluded following the IWGTP meeting in Washington in 1999 (Tice et al., 2000).”
• Comet assay using mullet (Mugil sp.) and sea catfish (Netuma sp.) erythrocytes for the detection of genotoxic pollutants in aquatic environment (2004, neutral): “The alkaline single cell gel electrophoresis assay, or comet assay, is a rapid, simple and sensitive procedure to quantify DNA lesions in individual cells for environmental monitoring [7,10–14].”