An ultrasensitive bacterial motor revealed by monitoring signaling proteins in single cells.

By P. Cluzel, Michael G. Surette, Stanislas Leibler. Science, 2000

609 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Cellular and Molecular Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a1cd607b6d25f03b4974663fca155d8f868229aa

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In Biomedical Engineering

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Citations in the corpus

Receptor clustering and signal processing in E. coli chemotaxis. (2004, background): “Another experiment, in which concentrations of fluorescently labelled CheY were determined in a single cell by fluorescence correlation spectroscopy (FCS), demonstrated a highly non-linear dependence of motor switching on CheY-P concentration, with a Hill coefficient of w10 [ 49 ].”
Fluorescence correlation spectroscopy: the technique and its applications (2002, methodology): “Cluzel et al (2000) used FCS to count fluorescent proteins in living cells.”
How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria (2006, background): “This could in turn slightly lower the amount of P CheY and lead to a strong chemotactic response, as the binding of P CheY to the flagellar motor is highly cooperative (133).”
Efficient modeling, simulation and coarse-graining of biological complexity with NFsim (2011, methodology): “We then used the model to fit experimental measurements of the single-cell motor response curv”
From signatures to models: understanding cancer using microarrays (2005, background): “This is a concern even when studying unicellular organisms or cell cultures as the averaging process tends to obscure variations between cells 53–55 .”
Anomalous diffusion of proteins due to molecular crowding. (2005, background): “Ær(2)ðtÞæ 1⁄4 6G t; (2) Submitted August 10, 2004, and accepted for publication August 3, 2005.”
Action at a Distance: Amino Acid Substitutions That Affect Binding of the Phosphorylated CheY Response Regulator and Catalysis of Dephosphorylation Can Be Far from the CheZ Phosphatase Active Site (2011, background): “The direction of rotation of the Escherichia coli flagellar motor, and hence the swimming behavior of the cell, exhibits a cooperative dependence on CheYp concentration with a Hill coefficient of about 10 and therefore is exquisitely sensitive to changes in CheYp (8).”
Studying protein dynamics in living cells (2001, background): “The amplitude of the AUTOCORRELATION FUNCTION of the fluctuations is inversely proportional to the average number of fluorescent objects and so constitutes an absolute in vivo measurement of the concentration of these objects in the confocal volum”