Fluorescence Silica Nanoprobe as a Biomarker for Rapid Detection of Plant Pathogens
By K. Yao, S.J. Li, K. Tzeng, T. Cheng, C. Chang, C. Chiu, C. Liao, J. Hsu, Z.P. Lin. 2009
156 citations recorded by the source corpus.
Fluorescent silica nanoprobe as a biomarker for detection has attracted much attention in the field of nano-biotechnology recently but no further research applications using fluorescent silica nanoparticles (FSNP) combined with antibody molecules reported to detect pathogen detection. In this study, silica nanoparticles were prepared using the water-in-oil (w/o) microemulsion method. The silica nanoparticles were circular in diameter of 50 ± 4.2 nm. The organic dye, tris-2, 2' -bipyridyl dichlororuthenium (II) hexahydrate (Rubpy), could be incorporated efficiently into the core of silica nanoparticles. The fluorescence of Rubpy-doped silica nanoparticles was photostable using a collisional quenching fluorescence test. The Rubpy-doped silica nanoparticles were conjugated with the secondary antibody of goat anti-rabbit immunoglobulin G (IgG) and successfully detected plant pathogen such as Xanthomonas axonopodis pv. vesicatoria that causes bacterial spot disease in Solanaceae plant. These results demonstrated that the fluorescence silica nanoprobe biomarker will have been potential for rapid diagnosis applications on plant diseases.
Subfields: Biomedical Engineering, Materials Chemistry, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/a1a89e4c05780c8f33baebe05d391132ca9ce2aa
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In Materials Chemistry
In Molecular Biology
Citations in the corpus
- Current and Prospective Methods for Plant Disease Detection (2015, methodology): “Nanoparticles have been utilized with other biological materials such as antibody for detecting Xanthomonas axonopodis that causes bacterial spot disease [65].”
- Nanoparticle-Based Sustainable Agriculture and Food Science: Recent Advances and Future Outlook (2020, methodology): “Fluorescence SiNPs, combined with Ab, were utilized to detect Xanthomonas axonopodis pv. vesicatoria, known to cause bacterial spot disease in Solanaceae plants (Yao et al., 2009).”
- Enhancement of the Antibacterial Activity of Silver Nanoparticles against Phytopathogenic Bacterium Ralstonia solanacearum by Stabilization (2016, background): “Previous studies have identified potential uses of nanotechnology in practically every field of agriculture, including the controlled release of fertilizers and micronutrients [10], pesticide degradation [11], biopesticide stabilization [12], nanosensing of plant pathogens and pesticides [13, 14], a”