Enhancement of the Antibacterial Activity of Silver Nanoparticles against Phytopathogenic Bacterium Ralstonia solanacearum by Stabilization

By Juanni Chen, Shili Li, Jinxiang Luo, Rong-Ye Wang, W. Ding. 2016

83 citations recorded by the source corpus.

In this paper, the enhanced antibacterial activity of silver nanoparticles (AgNPs) against the phytopathogenic bacterium Ralstonia solanacearum after stabilization using selected surfactants (SDS, SDBS, TX-100, and Tween 80) was examined, in comparison with silver ion. Tween 80 was found to be the most preferable stabilizer of AgNPs due to the beneficial synergistic effects of the AgNPs and surfactant. However, all the surfactants nearly had no effects on the antibacterial activity of Ag+. In vitro, Tween 80-stabilized AgNPs showed the highest bactericidal activity against R. solanacearum. Further measurements using TEM, fluorescence microscopy, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that though Ag+ and Tween 80-Ag+ induced high toxicity, Tween 80-stabilized AgNPs displayed most severe damage when in direct contact with cells, causing mechanistic injury to the cell membrane and strongly modifying and destructing the cellular proteins. Meanwhile, in vivo, the pot experiments data indicated that the control efficiency of Tween 80-stabilized AgNPs on tobacco bacterial wilt was 96.71%, 90.11%, and 84.21%, at 7 days, 14 days, and 21 days, respectively. Based on the results evidencing their advantageous low dosage requirements and strong antimicrobial activity, Tween 80-stabilized AgNPs are a promising antibacterial agent for use in alternative crop disease control approaches.

Subfields: Biomaterials, Materials Chemistry, Pollution

View Paper: https://www.semanticscholar.org/paper/fbc7e111bbcb91bca74497bef5ba0a269d3558b4

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Citations in the corpus

• Silver Nanoparticles and Their Antibacterial Applications (2021, methodology): “In relation to the size and charge of the nanoparticles, the stability of the products formed is also an important factor affecting the final antibacterial activity [52].”