Assessing soil biological characteristics : a comparison of bulk soil community DNA-, PLFA-, and Biolog-analyses

By F. Widmer, A. Fliessbach, E. Laczko, J. Schulze-Aurich, J. Zeyer. 2001

164 citations recorded by the source corpus.

Subfields: Environmental Chemistry, Molecular Biology, Soil Science

View Paper: https://www.semanticscholar.org/paper/a13a9dc5763d8f322bdad3565cb6b554e4fcfdcf

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In Environmental Chemistry

In Molecular Biology

In Soil Science

Citations in the corpus

Contrasting mass‐ratio vs. niche complementarity effects on litter C and N loss during decomposition along a regional climatic gradient (2017, background): “Although CLPP can track variation in microbial community structure as efficiently as PLFA (Widmer et al. 2001), some limitations may arise when applying to the decomposition process.”
Choice of Methods for Soil Microbial Community Analysis: PLFA Maximizes Power Compared to CLPP and PCR-Based Approaches (2006, unknown): “Independent assessments have indicated that each approach returns similar results with respect to the demonstration of rved. treatment effects (Widmer et al., 2001; Ritchie et al., 2000).”
Analysis of microbial community functional diversity using sole-carbon-source utilisation profiles - a critique. (2002, methodology): “Due to the number of caveats associated with community pro¢ling importance should be focussed on using BIOLOG observations in conjunction with other techniques, such as phospholipid fatty acid pro¢les (as done by, for example, [8,36]) and/or more traditional ecological methods.”
A Comparison of Soil Microbial Community Structure, Protozoa and Nematodes in Field Plots of Conventional and Genetically Modified Maize Expressing the Bacillus thuringiens is CryIAb Toxin (2005, methodology): “Widmer et al. (2001) also used both PLFA and CLPP to compare between soils, finding that the relative similarities of the soils varied according to the method and concluding that biological soil comparison was improved if more than one method was used.”
Functional and molecular responses of soil microbial communities under differing soil management practices (2004, methodology): “Nevertheless, CLPPs have been used to provide useful information for assessment of soil microbial community diversity (Baudoin et al., 2002; De Fede et al., 2001; O’Connel and Garland, 2002; Smalla et al., 1998; Widmer et al., 2001).”
Microbial community and functional diversity associated with different aggregate fractions of a paddy soil fertilized with organic manure and/or NPK fertilizer for 20 years (2015, unknown): “4.3 Microbial functional diversity Biolog plates, based on pure cultures of aerobic, heterotrophic microorganisms (Widmer et al. 2001), have commonly been used to assess the activity of bacterial communities.”
Response of soil microbial communities to compost amendments (2006, background): “…only indicate functional aspects of the cultivable fraction of the soil community that grows on the various carbon sources in the Biolog wells (Widmer et al., 2001) The comparison of the T-RFLP fingerprints among the different treatments revealed a big increase in the relative intensity of…”