Development of a BACarray translational profiling approach for the molecular characterization of CNS cell types
By M. Heiman, A. Schaefer, Shiaoching Gong, J. Peterson, Michelle Day, Keri E. Ramsey, M. Suárez‐Fariñas, C. Schwarz, D. Stephan, D. Surmeier and 2 more. 2008
1,118 citations recorded by the source corpus.
Summary The cellular heterogeneity of the brain confounds efforts to elucidate the biological properties of distinct neuronal populations. We have now developed a new ‘BACarray’ methodology, based on affinity purification of polysomal mRNAs from genetically defined cell populations. The utility of this approach is illustrated by the comparative analysis of four types of neurons, revealing hundreds of genes that distinguish these four cell populations. Even two morphologically indistinguishable subclasses of MSNs display vastly different translational profiles. Striatopallidal neurons are characterized by a strong and cell-specific release of intracellular Ca2+ in response to sphingosine 1-phosphate, consistent with their selective expression of Gpr6. In contrast, striatonigral neurons demonstrate a selective cell-specific increase in GABAA receptor subunits in response to chronic cocaine treatment. BACarray translational profiling is a generalizable method useful for the identification of molecular changes in any genetically defined cell type in response to genetic alterations, disease, or pharmacological perturbations.
Subfields: Molecular Biology, Radiology, Nuclear Medicine and Imaging
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
- E. Schuman
- Karl Deisseroth
- Charles R. Gerfen
- E. Sanz
- Liqun Luo
- Thomas Su
- Steven A. McCarroll
- G. Stanley Mcknight
In Radiology, Nuclear Medicine and Imaging
Citations in the corpus
- Cell-type-specific isolation of ribosome-associated mRNA from complex tissues (2009, methodology): “The Heintz laboratory has recently reported a similar approach (18, 19).”
- Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis (2009, background): “Epitope-tagged ribosomes were similarly captured to assess the remodeling of the translatome of yeast following mild and severe stress (16), and in discrete cell types in heterogeneous cultures of mouse neuronal cells (18).”
- Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain (2018, positive): “Expression of markers associated with canonical SPNs suggests eSPNs have been molecularly ‘‘camouflaged,’’ including in studies using mice that have employed Drd1- and Adora2a- driven transgenes to label and manipulate dSPNs or iSPNs (Heiman et al., 2008; Kozorovitskiy et al., 2012; Kravitz et al., ”
- Modulation of striatal projection systems by dopamine (2011, positive): “New techniques are revealing the extent of differences between direct and indirect SPNs (Lobo et al., 2006; Heiman et al., 2008; Meurers et al., 2009).”
- Wiring and Molecular Features of Prefrontal Ensembles Representing Distinct Experiences (2016, positive): “With this approach, the tagged ribosome can be immunoprecipitated (IP) from tissue lysate using an anti-GFP antibody, isolating translating mRNA for analysis (Ekstrand et al., 2014; Heiman et al., 2008; Sanz et al., 2009).”